MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) modulate gene expression. Along with several other classes of small noncoding RNAs (e.g. snRNA, snoRNA and piRNA), they have tremendous impact on a variety of biological processes.

While most traditional RNA isolation methods are not very efficient at recovering these smaller RNAs, several kits developed by our RNA experts offer quantitative recovery of small RNAs from a variety of sample types.

Which microRNA & small RNA isolation kit is right for you?

30 min isolation from most samples Recover all RNAs as well as proteins HTP isolation of total RNA enriched for small RNAs Complete kit, sample-to-qPCR from cells
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  mirVana™ miRNA Isolation Kit (with or without phenol) mirVana Paris MagMAX™ mirVana Total RNA Isolation Kit TaqMan® microRNA Cells-to-CT™ Kit
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RNA types isolated Small & large RNA molecules Small & large RNA molecules Small & large RNA molecules Small & large RNA molecules
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Prep time 20 minutes 30 minutes <1 hour 10 minutes
Compatible sample types Most sample types Most sample types Serum/Plasma, whole blood, Fresh/frozen tissue, cells, urine Cells
Isolation method Highest purity and convenience; includes both organic extraction and silica column Highest purity and convenience; includes both organic extraction and silica column Scalable, flexible format with magnetic beads Cells-to-CT™ direct lysis, no purification required
Reverse transcription reagents included No No No TaqMan® MicroRNA Reverse Transcription Kit
qPCR Master mix included No No No TaqMan®
Kit size 40 preps 40 preps 96 preps 100 lysis reactions - plus - reagents for 200 qRT-PCR assays

Publications

  • Johnson CD, Esquela-Kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, Wilson M, Wang X, Shelton J, Shingara J, Chin L, Brown D, Slack FJ. The let-7 MicroRNA Represses Cell Proliferation Pathways in Human Cells. Cancer Res. 2007 Aug 15;67(16):7713-22. [Abstract]
  • Shingara J, Keiger K, Shelton J, Laosinchai-Wolf W, Powers P, Conrad R, Brown D, Labourier E. An optimized isolation and labeling platform for accurate microRNA expression profiling.RNA. 2005 Jul 25; [Epub ahead of print] [Abstract]
  • Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A, Labourier E, Reinert KL, Brown D, Slack FJ. RAS is regulated by the let-7 microRNA family. Cell. 2005 Mar 11;120(5):635-47. [Abstract]
  • Cheng AM, Byrom MW, Shelton J, Ford LP. Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res. 2005 Mar 1;33(4):1290-7. [Abstract]

Resources

Technical Resources