Mammalian Transient Protein Expression

Get more protein faster- save 1 month of lab work.

Mammalian transient expression systems enable flexible and rapid production of proteins. They're ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more native folding and post-translational modifications—such as glycosylation—than expression systems based on hosts such as E. coli, yeast, or insect cells.

Choose the mammalian transient system that's right for you

	The most widely used transient expression system	Gram per liter protein yield 293-based system	Up to 3 g/L protein yield CHO-based system Most-cost effective
	FreeStyle™ 293 System	Expi293™ System	ExpiCHO™ System
Cells	FreeStyle™ 293-F	Expi293-F™	ExpiCHO-S™
Media	FreeStyle™ 293 Medium	Expi293™ Medium	ExpiCHO™ Medium
Transfection reagent	293Fectin™	ExpiFectamine™ 293 Transfection Kit	ExpiFectamine™ CHO Transfection Kit
Positive control vector	pCMV•Sport-βgal	Antibody-expressing vector	Antibody-expressing vector
Maximum working culture Density	2 x 10⁶/mL	6 x 10⁶/mL	10 x 10⁶/mL
Cell density for transfection	1 x 10⁶/mL	2.5 x 10⁶/mL	6 x 10⁶/mL
Protein yield*	20–200 mg/L	Up to 1 g/L	Up to 3 g/L
Order now	FreeStyle™ 293-F cells FreeStyle™ 293 Expression Medium 293Fectin™ Transfection Reagent pCMV•SPORT-βgal vector Opti-MEM® I FreeStyle™ 293 Expression System	Expi293-F™ cells Expi293™ Expression Medium ExpiFectamine™ Transfection Kit pcDNA 3.4 vector Antibody-expressing positive control vector Opti-MEM® I Expi293™ Expression System Kit	ExpiCHO™ Expression System Kit ExpiCHO-S™ Cells ExpiCHO™ Expression Medium ExpiFectamine™ CHO Transfection Kit pcDNA 3.4 vector Antibody-expressing positive control vector OptiPRO SFM