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Invitrogen NuPAGE Tris-Acetate Gels are high-performance gels that have been optimized for separating proteins of large molecular weight.
NuPAGE Tris-Acetate Gels are designed to give optimal separation of large molecular weight proteins during gel electrophoresis. In comparison to traditional tris-glycine SDS-PAGE gels, NuPAGE Tris-Acetate Gels have an environment of pH 8.1, which minimizes protein modifications and results in sharper bands.
Features of NuPAGE Tris-Acetate Gels include:
- High resolution—gels offer optimal separation of high molecular weight proteins
- Better protein integrity—sample preparation process has been optimized to help preserve your proteins
- Longer shelf life—gels can be stored for at least 8 months
NuPAGE Tris-Acetate Gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using LDS sample buffer and tris-acetate SDS running buffer. For native proteins, traditional tris-glycine native sample buffer and a tris-glycine native running buffer should be used.
The NuPAGE Tris-Acetate Gels use a discontinuous buffer system involving three ions:
- Acetate (–) is from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are tris (+) and acetate (–) (pH 7.0).
- Tricine (–) is from the running buffer and serves as the trailing ion. The running buffer ions are tris (+), tricine (–) and dodecylsulfate (pH 8.3).
- Tris (+) is the common ion present in the gel buffer and running buffer. The tris-acetate system also operates at a significantly lower operating pH of 8.1 during electrophoresis.
| Available gel sizes | Mini: 8 cm x 8 cm (1 or 1.5 mm thick) Midi: 8 cm x 13 cm (1 mm thick) |
| Storage conditions | Room temperature |
| Shelf life | 8 months |
| Recommended sample buffer | LDS sample buffer or native tris-glycine sample buffer |
| Recommended running buffers | Tris-acetate running buffer |
| Gel chemistry | Tris-acetate |
| Available polyacrylamide concentrations | 7%, 3–8% |
| Separation range (denaturing) | 30–500 kDa |
| For use with (equipment) mini gels | Mini Gel Tank or XCell SureLock Mini-Cell |
| For use with (equipment) midi gels | Bio-Rad Criterion (with adapters only) or Invitrogen XCell4 SureLock Midi-Cell |
| Mode of separation | Molecular weight |
Currently using Tris-glycine or Tricine gels? Use the NuPAGE Gel conversion guide below to find the appropriate NuPAGE gel and corresponding optimized buffer.
NuPAGE Gel conversion guide
| If you are using: | Then we recommend NuPAGE gel: | Buffer system |
|---|---|---|
| Tris-Glycine gel | ||
| 4% | NuPAGE 3-8% Tris-Acetate | Tris-Acetate |
| 6% | NuPAGE 3-8% Tris-Acetate | Tris-Acetate |
| 10% | NuPAGE 8% Bis-Tris | MOPS |
| NuPAGE 10% Bis-Tris | MOPS | |
| 12% | NuPAGE 10% Bis-Tris | MOPS |
| 14% | NuPAGE 12% Bis-Tris | MOPS |
| 16% | NuPAGE 12% Bis-Tris | MES |
| 18% | NuPAGE 12% Bis-Tris | MES |
| 4-12% | NuPAGE 3-8% Tris-Acetate | Tris-Acetate |
| NuPAGE 4-12% Bis-Tris | MOPS | |
| 4-20% | NuPAGE 4-12% Bis-Tris | MES |
| 8-16% | NuPAGE 4-12% Bis-Tris | MOPS |
| 10-20% | NuPAGE 12% Bis-Tris | MOPS |
| Tricine gel | ||
| 10% | NuPAGE 8% Bis-Tris | MES |
| NuPAGE 10% Bis-Tris | MES | |
| 16% | NuPAGE 4-12% Bis-Tris | MES |
| 10-20% | NuPAGE 4-12% Bis-Tris | MES |
NuPAGE Tris-Acetate Gels are designed to give optimal separation of large molecular weight proteins during gel electrophoresis. In comparison to traditional tris-glycine SDS-PAGE gels, NuPAGE Tris-Acetate Gels have an environment of pH 8.1, which minimizes protein modifications and results in sharper bands.
Features of NuPAGE Tris-Acetate Gels include:
- High resolution—gels offer optimal separation of high molecular weight proteins
- Better protein integrity—sample preparation process has been optimized to help preserve your proteins
- Longer shelf life—gels can be stored for at least 8 months
NuPAGE Tris-Acetate Gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using LDS sample buffer and tris-acetate SDS running buffer. For native proteins, traditional tris-glycine native sample buffer and a tris-glycine native running buffer should be used.
The NuPAGE Tris-Acetate Gels use a discontinuous buffer system involving three ions:
- Acetate (–) is from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are tris (+) and acetate (–) (pH 7.0).
- Tricine (–) is from the running buffer and serves as the trailing ion. The running buffer ions are tris (+), tricine (–) and dodecylsulfate (pH 8.3).
- Tris (+) is the common ion present in the gel buffer and running buffer. The tris-acetate system also operates at a significantly lower operating pH of 8.1 during electrophoresis.
| Available gel sizes | Mini: 8 cm x 8 cm (1 or 1.5 mm thick) Midi: 8 cm x 13 cm (1 mm thick) |
| Storage conditions | Room temperature |
| Shelf life | 8 months |
| Recommended sample buffer | LDS sample buffer or native tris-glycine sample buffer |
| Recommended running buffers | Tris-acetate running buffer |
| Gel chemistry | Tris-acetate |
| Available polyacrylamide concentrations | 7%, 3–8% |
| Separation range (denaturing) | 30–500 kDa |
| For use with (equipment) mini gels | Mini Gel Tank or XCell SureLock Mini-Cell |
| For use with (equipment) midi gels | Bio-Rad Criterion (with adapters only) or Invitrogen XCell4 SureLock Midi-Cell |
| Mode of separation | Molecular weight |
Currently using Tris-glycine or Tricine gels? Use the NuPAGE Gel conversion guide below to find the appropriate NuPAGE gel and corresponding optimized buffer.
NuPAGE Gel conversion guide
| If you are using: | Then we recommend NuPAGE gel: | Buffer system |
|---|---|---|
| Tris-Glycine gel | ||
| 4% | NuPAGE 3-8% Tris-Acetate | Tris-Acetate |
| 6% | NuPAGE 3-8% Tris-Acetate | Tris-Acetate |
| 10% | NuPAGE 8% Bis-Tris | MOPS |
| NuPAGE 10% Bis-Tris | MOPS | |
| 12% | NuPAGE 10% Bis-Tris | MOPS |
| 14% | NuPAGE 12% Bis-Tris | MOPS |
| 16% | NuPAGE 12% Bis-Tris | MES |
| 18% | NuPAGE 12% Bis-Tris | MES |
| 4-12% | NuPAGE 3-8% Tris-Acetate | Tris-Acetate |
| NuPAGE 4-12% Bis-Tris | MOPS | |
| 4-20% | NuPAGE 4-12% Bis-Tris | MES |
| 8-16% | NuPAGE 4-12% Bis-Tris | MOPS |
| 10-20% | NuPAGE 12% Bis-Tris | MOPS |
| Tricine gel | ||
| 10% | NuPAGE 8% Bis-Tris | MES |
| NuPAGE 10% Bis-Tris | MES | |
| 16% | NuPAGE 4-12% Bis-Tris | MES |
| 10-20% | NuPAGE 4-12% Bis-Tris | MES |
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