Séquençage de nouvelle génération (NGS)
Next-generation sequencing (NGS) is a high-throughput methodology that enables rapid sequencing of the base pairs in DNA or RNA samples. Supporting a broad range of applications, including gene expression profiling, chromosome counting, detection of epigenetic changes, and molecular analysis, NGS is driving discovery and enabling the future of personalized medicine.
Produits à la une
NGS research areas and applications
Produits & services
En savoir plus et commander les séquenceurs, puces, réactifs et accessoires Ion Torrent.
Publications
Lisez et téléchargez les publications Ion Torrent.
Chercheurs à la une
Regardez des vidéos de séquençage ciblé, d’exome, de transcriptome & de génome
Informatique de laboratoire
Exploitez au maximum vos données de séquençage par semi-conducteur avec des solutions bio-informatiques complètes.
Communauté
La communauté Ion Torrent est rédigée de manière collaborative par les personnes qui l’utilisent. Participez à la conversation !
Documentation
Consultez les notes d’application, les brochures et les dépliants.
Applications
PGM™pour les gènes. Proton™ pour les génomes. En savoir plus sur les applications Ion Torrent
Prestataires de services certifiés
Réseau de prestataires de services qui permet aux chercheurs d’obtenir des données de séquençage d’exome de la plus grande qualité.
Cancer research
From basic to translational to clinical research
Human identification (HID)
From genotyping to STRs to mitochondrial DNA
Reproductive health research
From pre-implantation to congenital research
Infectious disease
From viruses to microbial communities
Leading in NGS
What is Ion AmpliSeq Chemistry?
Ion Torrent targeted NGS solutions are built on industry-leading Ion AmpliSeq technology. What is AmpliSeq? Watch this 4 minute video to find out more.
Targeted NGS performance of Oncomine Assays and NCI-MATCH Trial Assay
The Oncomine Comprehensive Assay and NCI-MATCH Trial Assay (based on the Oncomine Comprehensive Assay) have demonstrated robust performance even with very limited FFPE samples. See the data ›
New to Sanger or NGS?
Build your NGS knowledge in the Sequencing Education Center.
Featured application: liquid biopsy research
From sample to answer, Ion Torrent technology enables comprehensive solutions for analysis from cell-free DNA (cfDNA).
Check out our new cfDNA white paper that discusses the analytical methods used in verification of the Oncomine Lung cfDNA Assay with the Ion S5 XL System.
Highlights:
- Our complete Oncomine cfDNA NGS workflow was used in this evaluation, including orthogonal verification with dPCR
- Samples evaluated include plasma, FFPE, hotspot controls, and reference standards
Data generated and analyzed demonstrate that the Ion Torrent NGS workflow using Oncomine cfDNA assays is reliable, and generates repeatable and reproducible results.
How does Ion Torrent NGS work?
Ion Torrent next-generation sequencing exploits the fact that addition of a dNTP to a DNA polymer releases a hydrogen ion. We measure the pH change resulting from those hydrogen ions using semiconductors, simultaneously measuring millions of such changes to determine the sequence of each fragment.
The semiconductor approach—unlike optics or modified nucleotides used in other NGS technologies—helps you implement a fast and simple workflow that scales to your research needs across multiple applications including inherited disease, oncology, infectious disease, human identification (HID), human leukocyte antigen (HLA) typing, and agrigenomics.
Our targeted sequencing approach introduces a PCR-based sequence enrichment step using Ion AmpliSeq technology that focuses on genes or even genetic variants of interest; a good example is the targeting of oncogenes and tumor suppressors in a cancer research study.
Custom Image: Double click to configure component
Custom Image: Double click to configure component
Support
Next-Generation Sequencing Support Center
Find tips, troubleshooting help, and resources for your next-generation sequencing applications.
For Research Use Only. Not for use in diagnostic procedures.