Whether you are culturing your cells on feeder layers or require feeder-free, xeno-free conditions for more clinical applications, Gibco products provide you with all the choices to culture your pluripotent stem cells in the right environment. W e provide extracellular matrices (ECMs) that are designed to:

  • Minimize adaptation time,
  • Maximize cell performance, and
  • Help meet regulatory requirements

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Which extracellular matrix is right for you?

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  Most commonly used basement membrane matrix for ESC and iPSC cultures Simplified solution for the most commonly used basement membrane Optimized for use with Essential 8 Medium Superior recovery during challenging transitions
Product name Geltrex LDEV-Free hESC Qualified Geltrex hESC Qualified, Ready-To-Use Vitronectin (VTN-N) Laminin 521
Recommended applications Feeder and feeder-free culture Feeder and feeder-free culture Feeder-free and xeno-free culture Feeder-free and xeno-free culture
Recommended medium pairing StemPro hESC SFM or StemFlex Medium StemPro hESC SFM or StemFlex Medium StemFlex Medium or Essential 8 Flex Medium StemFlex Medium or Essential 8 Flex Medium
Source origin Cultured mouse EHS tumor cells; undefined Cultured mouse EHS tumor cells; undefined Human (recombinant); defined Human (recombinant); defined
Storage temperature Frozen (-20 to -80° C) Refrigerate (2-8° C) Frozen (-20 to -80° C) Frozen (-20 to -80° C)
Reagent preparation time Thaw on ice, overnight in refrigerator, dilute 1:100 Ready to use Thaw at room temperature, dilute 1:100 Thaw at 2-8° C, dilute 1:50
Unit size 1 mL or 5 mL 50 mL 1 mL 1 mL or 10 mL
Number of plates coated/unit/span 16x6 well plate from 1 mL vial diluted 1:100 8x6 well plates 16x6 well plates from 1 mL vial diluted 1:100 3 x 6 well plate from 1 mL

Attachment Factor 0.1% Gelatin, (Cat. No. S006100) may be used for feeder-layer attachment.

For Research Use Only. Not for use in diagnostic procedures.