Greater fold enrichment than other commercially available kits

LNcaP prostate carcinoma cells were cultured in RPMI-1640 medium containing 10% FBS for 24 hours. Half of the cultures plated were treated for 16 hours with 5 µM camptothecin, a drug that inhibits DNA topoisomerase I. Crosslinking was achieved using a final concentration of 1% formaldehyde in the media for 10 minutes. Using kits from 4 different suppliers, ChIP assays were performed according to the manufacturers’ protocols to determine binding of p53 to a 1.5-kb region of the CDKN1A (p21) promoter. Quantitative real-time PCR was preformed. The Pierce Magnetic ChIP Kit has been optimized to isolate even large DNA fragments. Products compared: Thermo Scientific Pierce Magnetic ChIP Kit, Millipore Magna ChIP™ A/G, Cell Signaling Technology SimpleChIP™ Plus Enzymatic, Active Motif ChIP-IT™ Express Enzymatic Kit.

LNcaP prostate carcinoma cells were cultured in RPMI-1640 medium containing 10% FBS for 24 hours. Half of the cultures plated were treated for 16 hours with 5 µM camptothecin, a drug that inhibits DNA topoisomerase I. Crosslinking was achieved using a final concentration of 1% formaldehyde in the media for 10 minutes. Using kits from 4 different suppliers, ChIP assays were performed according to the manufacturers’ protocols to determine binding of p53 to a 1.5-kb region of the CDKN1A (p21) promoter. Quantitative real-time PCR was preformed. The Pierce Magnetic ChIP Kit has been optimized to isolate even large DNA fragments. Products compared: Thermo Scientific Pierce Magnetic ChIP Kit, Millipore Magna ChIP™ A/G, Cell Signaling Technology SimpleChIP™ Plus Enzymatic, Active Motif ChIP-IT™ Express Enzymatic Kit.

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