The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Invitrogen Alexa Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range.
The fluorescence quantum yield and high photostability in antifade allow detection of low-abundance biological structures in fixed cells with great sensitivity. Alexa Fluor 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.
We offer Alexa Fluor 680 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Alexa Fluor 680 dye dashboard
 Initial brightness |
Near IR dye provides signal in a spectral range easily separated from tissue autofluorescence. Useful for multiplexing western blot detection. In addition, useful in stochastic optical reconstruction microscopy (STORM) where it is used a reporter for dSTORM. | ||
 Photostability in buffer |
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 Photostability in antifade |
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 Performance in SRM |
| 633 | Cy5.5 | 679 | 702 | ||
 Laser line |
 Common filter set |
 Excitation max |
Alexa Fluor 680 products
We offer a broad range of Alexa Fluor 680 products including labeling kits, secondary antibodies, bioconjugates, and more:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits for direct detection
- Secondary antibodies for flexible labeling
- Streptavidin conjugates for signal amplification
- Tyramide labeling kits for ultimate sensitivity
- Phalloidin conjugates for actin staining
- Annexin V conjugates for apoptosis imaging
- Labeled nucleotides for hybridization probes
Golgi in fixed and permeabilized NIH 3T3 mouse fibroblast cells was labeled with green-fluorescent Invitrogen Alexa Fluor 488 conjugate of HPA lectin. Mitochondria were labeled with a primary antibody directed against OxPhos Complex V Inhibitor Protein and visualized using far-red-fluorescent Alexa Fluor 680 goat anti-mouse IgG. Filamentous actin was stained with red-orange–fluorescent Invitrogen Alexa Fluor 568 phalloidin. Nuclei were stained with blue-fluorescent DAPI.
Alexa Fluor 680 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor 680 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg mouse IgG1 | Zenon Alexa Fluor 680 Mouse IgG1 Labeling Kit | Z25010 |
| <1–20 µg mouse IgG2a | Zenon Alexa Fluor 680 Mouse IgG2a Labeling Kit | Z25110 |
| <1–20 µg mouse IgG2b | Zenon Alexa Fluor 680 Mouse IgG2b Labeling Kit | Z25210 |
| Conventional antibody/protein labeling | ||
| 100 µg antibody/protein | Alexa Fluor 680 Antibody/Protein Labeling Kit | A20188 |
| 1 mg antibody/protein | Alexa Fluor 680 Antibody/Protein Labeling Kit | A20172 |
| 0.5–3 mg antibody | Alexa Fluor SAIVI 680 Antibody Labeling Kit | S30045 |
Alexa Fluor dye family
Illuminating research for over 15 years
Invitrogen Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Molecular Probes Handbook
Resources
Molecular Probes Handbook
Antifade reagents
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
Learn more about SlowFade Antifade reagents
Learn more about ProLong Antifade reagents
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.