AccuPrime DNA Polymerases
Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime™ technology is available in Taq, Pfx, GC-Rich, and SuperMix formulations.
Overview of AccuPrime Technology
The thermostable AccuPrime accessory proteins enhance specific primer–template hybridization during every cycle of PCR, preventing mispriming and improving PCR specificity and yield. In addition, AccuPrime enzymes are designed with Platinum antibodies that are specific to the DNA polymerase and inhibit its activity at room temperature, providing an automatic hot start.
AccuPrime DNA polymerase products
|AccuPrime Taq DNA Polymerase, High Fidelity||AccuPrime Pfx DNA Polymerase||AccuPrime GC-Rich DNA Polymerase||AccuPrime Taq DNA Polymerase System||AccuPrime SuperMix I||AccuPrime SuperMix II|
|DNA polymerase(s)||Blend of proofreading enzyme from Pyrococcus species GB-D and Taq DNA Polymerase||AccuPrime Pfx DNA Polymerase||AccuPrime GC-Rich DNA Polymerase cloned from Pyrolobus fumarius||AccuPrime Taq DNA Polymerase||AccuPrime Taq DNA Polymerase||AccuPrime Taq DNA Polymerase|
|Platinum hot-start technology||✓||✓||✓||✓||✓|
|Enhanced specificity with AccuPrime accessory proteins||✓||✓||✓||✓||✓||✓|
|Fidelity (compared to Taq polymerase)||9x||26x||2x||2x||2x||2x|
|Resulting amplicon end||3′-A, mixed||Blunt||Blunt||3′-A||3′-A||3′-A|
|Formats available||Stand-alone enzyme with the two-buffer system. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for gDNA.||Stand-alone enzyme||Stand-alone enzyme with the two-buffer system. Buffer A is optimized for GC-rich gDNA targets. Buffer B is optimized for non–GC-rich gDNA, cDNA, plasmids, and λ DNA.||Stand-alone enzyme with two buffer system. Buffer I is designed for small gDNA amplicons (≤200 bp), cDNA, and plasmids. Buffer II is designed for gDNA (200 bp–4 kb).||SuperMix I||SuperMix II|
|Applications||Long PCR, multiplex PCR up to 15-plex||Cloning, mutagenesis||Amplification of difficult (GC-rich) templates||Multiplex PCR, allele-specific amplifications||Multiplex PCR, allele-specific amplifications||Multiplex PCR, allele-specific amplifications|