TATA Binding Protein Antibody (MA1-189) in IF

Immunofluorescent analysis of TATA binding protein (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product ## 37525) for 30 minutes at room temperature. Cells were stained with a TBP monoclonal antibody (Product ## MA1-189) at a dilution of 10ug/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product ## A28175) at a dilution of 1:250 for 1 hour at room temperature (green). Nuclei (blue) were counterstained with Hoechst 33342 dye (Product ## 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Immunofluorescent analysis of TATA binding protein (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product ## 37525) for 30 minutes at room temperature. Cells were stained with a TBP monoclonal antibody (Product ## MA1-189) at a dilution of 10ug/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product ## A28175) at a dilution of 1:250 for 1 hour at room temperature (green). Nuclei (blue) were counterstained with Hoechst 33342 dye (Product ## 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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