Figure 4. Comparison of inhibitor tolerance of TaqPath 1-Step Multiplex Master Mix and competitor kits

Two inhibitors of RT-PCR (hematin and heparin) were added to real-time PCR reactions run using the QuantStudio™ 5 Real-Time PCR System to assess the magnitude of Ct shift caused by these inhibitors. Graphs show the change in Ct from the baseline value with no inhibitor present. B = Bio-Rad iTaq Universal Probes One-Step Kit. Q1 = Qiagen QuantiTect Virus Kit. Q2 = Quanta Bioscience qScript XLT One-Step Tough Mix.

Two inhibitors of RT-PCR (hematin and heparin) were added to real-time PCR reactions run using the QuantStudio™ 5 Real-Time PCR System to assess the magnitude of Ct shift caused by these inhibitors. Graphs show the change in Ct from the baseline value with no inhibitor present. B = Bio-Rad iTaq Universal Probes One-Step Kit. Q1 = Qiagen QuantiTect Virus Kit. Q2 = Quanta Bioscience qScript XLT One-Step Tough Mix.

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