P-Cadherin Antibody (MA324000A555) in IF

Immunofluorescent analysis of p-Cadherin (orange) in A431 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product ## 37525) for 30 minutes at room temperature. Cells were stained with a p-Cadherin mouse monoclonal antibody, AlexaFluor 555 conjugate (Product ## MA324000A555), at a concentration of 10ug/ml in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product ## 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Immunofluorescent analysis of p-Cadherin (orange) in A431 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product ## 37525) for 30 minutes at room temperature. Cells were stained with a p-Cadherin mouse monoclonal antibody, AlexaFluor 555 conjugate (Product ## MA324000A555), at a concentration of 10ug/ml in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product ## 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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