Detection with 1-Step Ultra TMB-Blotting Solution and other commercial TMB substrates

Serial dilutions of HepG2 cell lysate (15, 7.5, 3.45, 1.88, 0.94μg) were prepared and separated by electrophoresis. The proteins were transferred to nitrocellulose (TOP) membranes (Part No. 88013) and PVDF (BOTTOM) membranes (Part No. 88518). The membranes were blocked with Clear Milk Blocker 1X (Part No. 37587). After blocking, the membranes were incubated with mouse PLK-1 Monoclonal Antibody (Part No. MA1-848) and Cyclophilin B Polyclonal Antibody (Part No. PA1-027A). The membranes were washed and then incubated with 0.2μg/mL of HRP-Conjugated Goat Anti-Mouse IgG (Part No. 31430) and HRP-Conjugated Goat Anti-Rabbit IgG (Part No. 31460) and then washed again. The membranes were placed in 10mL of Pierce 1-Step Ultra TMB-Blotting Solution (Part No. 37574) (A), TMB substrate from Supplier B. The color development was stopped at 3 minutes (BOTTOM) and 5 minutes (TOP) by rinsing the membranes with ultra pure water.

Serial dilutions of HepG2 cell lysate (15, 7.5, 3.45, 1.88, 0.94μg) were prepared and separated by electrophoresis. The proteins were transferred to nitrocellulose (TOP) membranes (Part No. 88013) and PVDF (BOTTOM) membranes (Part No. 88518). The membranes were blocked with Clear Milk Blocker 1X (Part No. 37587). After blocking, the membranes were incubated with mouse PLK-1 Monoclonal Antibody (Part No. MA1-848) and Cyclophilin B Polyclonal Antibody (Part No. PA1-027A). The membranes were washed and then incubated with 0.2μg/mL of HRP-Conjugated Goat Anti-Mouse IgG (Part No. 31430) and HRP-Conjugated Goat Anti-Rabbit IgG (Part No. 31460) and then washed again. The membranes were placed in 10mL of Pierce 1-Step Ultra TMB-Blotting Solution (Part No. 37574) (A), TMB substrate from Supplier B. The color development was stopped at 3 minutes (BOTTOM) and 5 minutes (TOP) by rinsing the membranes with ultra pure water.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›