MUSK Antibody (PA1-1741) in IHC (P)

Immunohistochemistry was performed on mouse skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes and then probed with (right panel) or without (left panel) a MuSK polyclonal antibody (Product ## PA1-1741) at a dilution of 1:20 overnight at 4C in a humidified chamber. Tissues were washed extensively with TBS + 0.025% Triton X-100 (Product ## 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product ## 35000) for 30 minutes at room temperature. Detection was performed using a goat anti-rabbit HRP secondary antibody (Product ## 31460) followed by colorimetric detection using metal enhanced DAB (Product ## 34065). Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry was performed on mouse skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes and then probed with (right panel) or without (left panel) a MuSK polyclonal antibody (Product ## PA1-1741) at a dilution of 1:20 overnight at 4C in a humidified chamber. Tissues were washed extensively with TBS + 0.025% Triton X-100 (Product ## 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product ## 35000) for 30 minutes at room temperature. Detection was performed using a goat anti-rabbit HRP secondary antibody (Product ## 31460) followed by colorimetric detection using metal enhanced DAB (Product ## 34065). Tissues were counterstained with hematoxylin and prepped for mounting.

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