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GPR18 Antibody (PA3-006) in WB
Western blot analysis of GPR18 was performed by loading equal amounts of wheat germ lectin agarose bead enriched GPR receptor fractions from GPR18 or GPR3 transfected HEK293 lysates and 10ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product ## 62288), and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product ## 37543) for 1 hour at room temperature. GPR18 was detected at ~70kDa using a GPR18 polyclonal antibody (Product ## PA3-006) at a dilution of 1:1000 in StartingBlock T20 (TBS) Blocking Buffer (Product ## 37543) overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product ## 31460) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product ## 34075). Images were acquired on a Thermo Scientific myECL Imager (Product ## 62236).
