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Tetramethylrhodamine (TRITC)
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| 4 | 488/532 | TRITC | 522 | 578 | (in buffer) 4 (in antifade) 5 | microscopy, flow cytometry, fluorescence microplate reader |
Invitrogen Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation well-suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications. TRITC is extensively used to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates.
For brighter conjugates and greater photostability, we offer a Texas Red dye alternative—Alexa Fluor 555—as a reactive dye or conjugated to a variety of antibodies, peptides, proteins, tracers or amplification substrates.
A wide variety of TRITC products, including antibodies and bioconjugates, are available that have been optimized for cellular labeling and detection. In addition, we offer reactive dyes that allows you to generate your own antibody conjugates or probes.
Search TRITC secondary antibodies Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)
Muntjac skin fibroblast labeled with Rhodamine phalloidin. The actin cytoskeleton of a fixed and permeabilized muntjac skin fibroblast was labeled with Rhodamine phalloidin; the nucleus was stained with DAPI. The sample was mounted and imaged in SlowFade Gold Antifade Mountant.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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