Technical Resources

32360 - MEM, HEPES, no glutamine

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
L-Arginine hydrochloride	211.0	126.0	0.5971564
L-Cystine	240.0	24.0	0.1
L-Histidine hydrochloride-H2O	210.0	42.0	0.2
L-Isoleucine	131.0	52.0	0.39694658
L-Leucine	131.0	52.0	0.39694658
L-Lysine hydrochloride	183.0	73.0	0.3989071
L-Methionine	149.0	15.0	0.10067114
L-Phenylalanine	165.0	32.0	0.19393939
L-Threonine	119.0	48.0	0.40336135
L-Tryptophan	204.0	10.0	0.04901961
L-Tyrosine	181.0	36.0	0.19889502
L-Valine	117.0	46.0	0.3931624
Vitamins
Choline chloride	140.0	1.0	0.007142857
D-Calcium pantothenate	477.0	1.0	0.002096436
Folic Acid	441.0	1.0	0.0022675737
Niacinamide	122.0	1.0	0.008196721
Pyridoxal hydrochloride	204.0	1.0	0.004901961
Riboflavin	376.0	0.1	2.6595744E-4
Thiamine hydrochloride	337.0	1.0	0.002967359
i-Inositol	180.0	2.0	0.011111111
Inorganic Salts
Calcium Chloride (CaCl2-2H2O)	147.0	264.0	1.7959183
Magnesium Sulfate (MgSO4-7H2O)	246.0	200.0	0.8130081
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2200.0	26.190475
Sodium Chloride (NaCl)	58.0	6300.0	108.62069
Sodium Phosphate monobasic (NaH2PO4-2H2O)	156.0	158.0	1.0128205
Other Components
D-Glucose (Dextrose)	180.0	1000.0	5.5555553
HEPES	238.0	5960.0	25.042017
Phenol Red	376.4	10.0	0.026567481

Reference:

1. Eagle, H. (1959) Science 130:432.