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VeriFiler Plus and VeriFiler Express PCR Amplification kits are designed to meet challenging forensic casework and databasing needs globally. Utilizing our 6-dye STR chemistry, the VeriFiler kits contain a total of 25 markers—23 autosomal STRs, including Penta D and Penta E, and two gender discrimination markers. The kits are manufactured in our ISO18385 certified facility and are supported by our comprehensive training, service, and applications teams.

With 11 mini-STRs, VeriFiler Plus kits are specifically designed for challenging sample types: touch, inhibited, or degraded samples, with improved sensitivity and robustness to inhibition. In addition, an Internal Quality Control (IQC) system assesses for the presence of inhibitors in the sample to enable distinction between degraded and inhibited samples which can help forensics analysts make better decisions on how to further process problematic samples. The IQC system also verifies PCR amplification.

VeriFiler Express kits offer superior discrimination power and high throughput, with efficient processing from single source samples to profile in as little as two hours. They have increased flexibility for use with multiple sample collection devices and there are protocols available for the most commonly used substrates.

Allele size comparison for VeriFiler Plus kit (left) and VeriFiler Express kit (right)

 

VeriFiler Plus kit

Verifiler Express kit

High discrimination power

25-marker multiplex assay that includes two gender markers as well as the highly discriminatory Penta D and Penta E markers

25-marker multiplex assay that includes two gender markers as well as the highly discriminatory Penta D and Penta E markers

Improved sensitivity

Kit allows for loading up to 17.5 µL of sample volume per 25 µL reaction

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Optimized chemistry

Optimized chemistry for challenging sample types: touch, inhibited, or degraded samples

Validated with multiple sample collection devices such as treated paper, untreated paper, and swabs; designed to work with the most commonly used substrates

Internal Quality Control system

Yes: for distinguishing inhibited and degraded samples; positive control for PCR amplification

No

DNA input

500 pg purified DNA, or up to 17.5 µL of sample volume

Treated or untreated paper: 1.2 mm punch

Swab: 2 µL (of 400 µL) Prep-n-Go buffer

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Need help bringing products online?

Look no further than our Human Identification Professional Services (HPS)  team. Since 2007, we have completed over 400 successful validation projects worldwide with a team of more than 20 technical support specialists, each averaging eight years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

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If you have additional comments or questions, please contact the customer service team via email.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing