Sall4 Antibody (720030) in IF

Immunofluorescent analysis of Sall4 (red) in human ESCs WIRB3 and HES2. The cells were fixed with 300 uL 4% paraformaldehyde solution for 15 min at RT in the dark, permeabilized with 300 uL 0.25% TritonX-100 solution (diluted in PBS) for 5 min at RT, and blocked with 300 uL 10% BSA solution (diluted in PBS) for 30 min in a 37C incubator. Cells were stained with a Sall4 polyclonal antibody (Product # 720030) at a dilution of 1:250 (2ug/ml) in 3% BSA solution (diluted in PBS) at 4C overnight, and then incubated with a secondary antibody (Red). Nuclei (blue) was stained with DAPI (Product # D1306). Images were taken on a Leica DM5500 microscope at 10X magnification. Data courtesy of Dr. Jianlong Wang's lab.

Immunofluorescent analysis of Sall4 (red) in human ESCs WIRB3 and HES2. The cells were fixed with 300 uL 4% paraformaldehyde solution for 15 min at RT in the dark, permeabilized with 300 uL 0.25% TritonX-100 solution (diluted in PBS) for 5 min at RT, and blocked with 300 uL 10% BSA solution (diluted in PBS) for 30 min in a 37C incubator. Cells were stained with a Sall4 polyclonal antibody (Product # 720030) at a dilution of 1:250 (2ug/ml) in 3% BSA solution (diluted in PBS) at 4C overnight, and then incubated with a secondary antibody (Red). Nuclei (blue) was stained with DAPI (Product # D1306). Images were taken on a Leica DM5500 microscope at 10X magnification. Data courtesy of Dr. Jianlong Wang's lab.

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