Low-picogram to high-femtogram detection

Turbo GFP-His-HA-Flag was diluted in electrophoresis reducing sample buffer. Lane 1 contained 10 pg of the purified protein with serial dilutions prepared 1:1 and applied at 10 µL/well. After electrophoresis, proteins were transferred to nitrocellulose membrane (Cat. No. 88018) using the Pierce™ Power Blotter (Cat. No. 22834) and Pierce 1-Step Transfer Buffer (Part No. 84731). The membrane was blocked with SuperBlock in TBST Buffer (Part No. 37536). Then the membrane was incubated with Anti-His (Part No. MA1-21315) at 1 µg/mL, followed by incubation with Goat anti-Mouse Horseradish Peroxidase Conjugate (Part No. 32430) at 100 ng/mL. SuperSignal West Pico PLUS substrate (Part No. 34577) was used for detection. A thirty second exposure was acquired on the Thermo Scientific MYECL Imager (Part No. 62236) and the image was inverted and contrasted (black = 55,000, white = 65,535, gamma = 1.0).

Turbo GFP-His-HA-Flag was diluted in electrophoresis reducing sample buffer. Lane 1 contained 10 pg of the purified protein with serial dilutions prepared 1:1 and applied at 10 µL/well. After electrophoresis, proteins were transferred to nitrocellulose membrane (Cat. No. 88018) using the Pierce™ Power Blotter (Cat. No. 22834) and Pierce 1-Step Transfer Buffer (Part No. 84731). The membrane was blocked with SuperBlock in TBST Buffer (Part No. 37536). Then the membrane was incubated with Anti-His (Part No. MA1-21315) at 1 µg/mL, followed by incubation with Goat anti-Mouse Horseradish Peroxidase Conjugate (Part No. 32430) at 100 ng/mL. SuperSignal West Pico PLUS substrate (Part No. 34577) was used for detection. A thirty second exposure was acquired on the Thermo Scientific MYECL Imager (Part No. 62236) and the image was inverted and contrasted (black = 55,000, white = 65,535, gamma = 1.0).

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