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NOX3 Antibody (PA5-38036) in IF
Immunofluorescent analysis of Nox3 (Red) in human G-CSF mobilized CD34+ cells. The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product ## PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Dylight 594 (Product ## 35560) at a dilution of 1:1000 for 1 hours at RT (panel b: red) and wash 5 times with 1xPBS before overlaid with cover slip. Nuclei (panel a: blue) were stained with DAPI (Product ## D1306) during the blocking step. Panel c was the bright field. Panels a, b and c are a merged image and magnified squire area, respectively. Images were taken on a Nikon A1 confocal microscopy at 60x Objective lens with oil used. Data courtesy of Dr. Zhi Wen from the University of Wisconsin, Madison.
