Detecting changes in cellular redox potential in HepG2 cells using Premo™ Cellular Redox Sensor

HepG2 cells were plated on collagen 1-coated 35 mm MatTek dishes at a density of 75,000 cells and transduced with Premo™ Cellular Redox Sensor. After 48 hrs, time lapse imaging was done on a confocal microscope using 400 nm and 488 nm lasers for excitation with the emission at 515 nm. The images were taken every 15 seconds with or without addition of 50 µM hydrogen peroxide for 10 minutes. Fluorescence intensity values were quantified by making regions of interest on the cells and were used to calculate 400/488 nm excitation ratios. The ratios were plotted against time.

HepG2 cells were plated on collagen 1-coated 35 mm MatTek dishes at a density of 75,000 cells and transduced with Premo™ Cellular Redox Sensor. After 48 hrs, time lapse imaging was done on a confocal microscope using 400 nm and 488 nm lasers for excitation with the emission at 515 nm. The images were taken every 15 seconds with or without addition of 50 µM hydrogen peroxide for 10 minutes. Fluorescence intensity values were quantified by making regions of interest on the cells and were used to calculate 400/488 nm excitation ratios. The ratios were plotted against time.

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