Our selection of first strand cDNA synthesis kits includes either RevertAid or Maxima Reverse Transcriptases (RT). RevertAid is genetically modified M-MuLV RT and offers routine performance. Maxima RT was developed by in vitro evolution of M-MuLV enzyme for superior performance in cDNA synthesis.

Thermo Scientific cDNA synthesis kit selection table


Routine performance

Enhanced performance

  RevertAid H Minus First Strand cDNA Synthesis Kit RevertAid First Strand cDNA Synthesis Kit Maxima H Minus First Strand cDNA Synthesis Kit Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Optimal reaction temperature 42-45°C 42°C 50-55°C 50-55°C
Active up to 55°C 50°C 65°C 65°C
Reading length up to 13 kb 13 kb 20 kb 20 kb
RNase H activity No Yes No Yes
Formats with dsDNase*     Yes Yes
Sensitivity (total RNA) 0.1 ng 0.1 ng 1 pg 1 pg
Reaction time 60 min 60 min 15-30 min 15-30 min

*Allow quick (2 min) and easy gDNA removal prior to reverse transcription step

Additional cDNA synthesis kits


Find videos, webinars, articles, and tools in our Molecular biology resource library

Reverse transcription content