Kits developed to quantify RNA in a one step assay. Kits for both SYBR Green and probe chemistry are available offering a simple way to perform rapid, sensitive and reproducible RT-qPCR in a single tube.

Thermo Scientific RT-qPCR kit selection table

  Probe Chemistry SYBR Green Chemistry
  Verso 1-Step RT-qPCR Kit Verso 1-Step RT-qPCR SYBR Green Kit
Optimal reaction temperature 50°C 50°C
Active up to 57°C 57°C
Blue dye - pipetting aid ✓ ✓
Sensitivity (total RNA) 1 pg 1 pg
Master Mix without ROX
(separate ROX vial)		   Yes
Master Mix with Low ROX Yes Yes
Master Mix with High ROX Yes  
RT enhancer* ✓ ✓

*Prevents gDNA carry-over

Resources

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Reverse transcription content

  

 

For Research Use Only. Not for use in diagnostic procedures.