Talk TitleSpeakerAbstract Duration
Divide and Conquer – Digital PCR-Based Detection of EGFR Mutations in Lung Cancer Patients using cell-free DNADr. MinGin KimEpidermal growth factor receptor (EGFR) mutations can drive cancers and cause resistance to treatments in patients with non-small cell lung cancer (NSCLC). Identifying EGFR mutations in patient cell-free DNA (cfDNA) may enable researchers to measure therapeutic response, quantify residual tumor burden, monitor emerging resistance to therapies, and early prediction recurrence up to a year. Here, we present 3 multiplex EGFR mutation panels compatible with cfDNA for use on the Absolute QTM digital PCR system which allows absolute and accurate quantification of the target sequences. We demonstrate the application of Absolute Q with the 3 EGFR mutation panels to detect multiple mutations with high sensitivity (0.1 % allele frequency, AF) simultaneously, thus enabling liquid biopsy cancer research for early detection and monitoring.20 mins
The Many Expressions of Cancer Genes –  Gene Expression qPCR Analysis of Cancer GenesJonathan FlasherAlthough there are several cancers that stem from a single mutation or deletion of a gene there are many more cancers that stem from genes that are not altered on the DNA level, but are altered in how they are expressed, leading to the dysregulation and progression of that cancer.  For these Class II genes, gene expression testing is not just a way to explore the pathophysiology of the cancers they cause, but also how they can be treated.  In this presentation we explain how TaqMan qPCR Gene Expression assays can be used to understand the phenotypic variability of the expression of genes that can cause cancer, understand the prognosis of certain cancers, and explore potential candidates for treatment.  20 mins