- Binding to Fc receptors on target cells
- Nonspecific antibody interactions with cellular proteins, carbohydrates, and lipids
- Cellular autofluorescence
Find isotype controls
Selecting an isotype control
Isotype controls should be matched to the primary antibody’s host species and class, including light chains (e.g., IgG2 Lambda, IgA1 Kappa). If using directly labeled primary antibodies, it is important to use the same conjugate to avoid differences in detection.
Applications for isotype controls
Although isotype controls are mainly used in flow cytometry, they can be used as standard blocking agents and protein coating agents for other applications including immunofluorescence, immunocytochemistry, western blotting, and ELISA.
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For Research Use Only. Not for use in diagnostic procedures.