Fluorescent Secondary Antibodies
Fluorescent-dye conjugated secondary antibodies provide a much needed tool for identifying proteins in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled secondary antibody include brighter signal, multiplexing capabilities, and ease of use (many are available for sale pre-conjugated to many different color of dyes). We offer a wide selection of fluorescent-dye conjugated secondary antibodies for your research, with over 60 different fluorescent dyes and dye combinations, and nanocrystals in a wide range specific target and host species.
How to find a secondary antibody
Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.
Quick links to secondary antibody conjugate products
|Enzyme & Biotin-Labeled Secondaries|
|NEW Alexa Fluor Plus Secondary Antibodies – an innovation in secondary antibodies offering higher signal-to-noise ratio for your precious samples.|
Compare fluorophore conjugate technologies
See how classical fluorophore, Alexa Fluor, and Alexa Fluor Plus secondary antibody conjugates compare.
|Alexa Fluor Plus
|What are they?||Affordable, traditional dyes conjugated to secondary antibodies||Bright and stable industry-leading dyes conjugated to secondary antibodies||Innovative, proprietary dyes conjugated to highly cross-adsorbed secondary antibodies|
|Photostability||Varying levels of photostability, depending on dye||Photostable||Photostable|
|Specificity||Various options available||Various options available||Highly specific, low cross-reactivity (highly cross-adsorbed)|
|Sensitivity and brightness||Lower sensitivity and brightness compared to leading Alexa Fluor conjugates||Widely recognized standard for brightness and sensitivity||Improved sensitivity and brightness over leading Alexa Fluor conjugates in IF/ICC imaging and fluorescent western blotting|
|Research areas||Designed for detection of high-abundance targets in research areas with easy-to-obtain samples||Designed for detection of medium- to high-abundance targets in most research areas||Designed for detection of low-abundance targets in research areas with hard-to-obtain samples, e.g., neuroscience|
|Range of linear detection||Low||Medium||High|
Featured secondary antibody conjugate categories
Select from 18 different individual Alexa Fluor dyes and 4 Alexa Fluor tandem dyes as secondary antibody conjugates. Alexa Fluor secondary antibodies exhibit superior brightness and photostability, outperforming conventional fluorescent secondary antibodies.
Learn about Alexa Fluor Plus secondary antibodies, which represent an advancement in fluorescent conjugate technology, designed to provide brighter signal, enhanced sensitivity, and minimal cross-reactivity in a variety of applications.
Find secondary antibody conjugates of traditional fluorophores, such as fluorescein (FITC), tetramethyl-rhodamine (TRITC), Cy3, Cy5, Pacific, and Texas Red dyes.
Discover features and varieties of available horseradish peroxidase (HRP), alkaline phosphatase (AP), biotin, and other enzyme or affinity-tagged secondary antibody conjugates.
For Research Use Only. Not for use in diagnostic procedures.