Antibody Labeling Kits

Molecular Probes® Antibody Labeling Kits label 100 µg IgG polyclonal and monoclonal antibodies with our superior Alexa Fluor®, Pacific Blue™, and Pacific Orange™ dyes.

  • Simple—labeled antibodies ready in 90 minutes requiring approximately 15 minutes actual hands-on time
  • Reliable—protein conjugates with stable attachment of fluorescent labels for subsequent use in fluorescence-based imaging or flow cytometry
  • Convenient—eliminates background staining commonly observed with secondary antibodies and allows more than one same-species antibody in a single experiment

Molecular Probes® Antibody Labeling Kit selection guide

Fluorophore
Ex/Em
Flow cytometry
Imaging
Cat. No.
Alexa Fluor® 350
346/442
  
Alexa Fluor® 488
495/519
Alexa Fluor® 532
532/553
  
A20182
Alexa Fluor® 546
556/573
  
A20183
Alexa Fluor® 555
555/565
  
A20187
Alexa Fluor® 568
578/603
  
A20184
Alexa Fluor® 594
590/617
  
A20185
Alexa Fluor® 647
650/665
A20186
Alexa Fluor® 680
684/707
A20188
Alexa Fluor® 790
784/814
  
A20189
Pacific Blue™ dye
410/455
P30013
Pacific Orange™ dye
405/551
 
P30014
Ex/Em = Fluorescence excitation and emission maxima, in nm.

How Molecular Probes® antibody labeling kits work

The Antibody Labeling Kits utilize an amine-reactive Alexa Fluor®, Pacific Blue™ or Pacific Orange™ fluorophore to covalently attach the label to the IgG antibody of interest.  Once formed, the covalent bond between the label and the protein is extremely stable and you are using the same chemistry we use to prepare our own primary and secondary conjugates.

These kits are optimized for labeling 100 µg of antibody per reaction. Comparably small amounts of other proteins (>40 kDa) can also be labeled. Simply adjust the protein concentration to ~1 mg/mL in the provided buffer, then add it to a vial of amine-reactive dye. Any stabilizing proteins (i.e., BSA) must be removed prior to the labeling reaction.

Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins (Figure 1). The entire labeling and purification procedure can be completed in as little as 90 minutes and everything needed to perform five conjugations is provided.

   Molecular Probes® Antibody Labeling Kits
Figure 1. Simply adjust the protein concentration to ~1 mg/mL in the provided buffer, then add it to a vial of amine-reactive dye. Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins. 		 

Need to label other antibody amounts?

IgG amount Attachment of label Products
<1–20 µg Site-specific Zenon® Antibody Labeling Kits
<100 µg Site-specific SiteClick™ Antibody Labeling Kits
10–20 µg Conventional covalent APEX® Antibody Labeling Kits
20–100 µg Conventional covalent Microscale Protein Labeling Kits
100 µg Conventional covalent Antibody Labeling Kits
1 mg Conventional covalent Protein Labeling Kits
0.5–3 mg Conventional covalent SAIVI™ Rapid Antibody Labeling Kits
Resources

Research Tools

BioProbes® Journal article

Molecular Probes® Handbook

For Research Use Only. Not for use in diagnostic procedures.