GeneArt® Precision TALs: Precise Genome Engineering
GeneArt® Precision TALs provide custom DNA-binding proteins for accurate DNA targeting and precise genome editing. Other technologies limit the choice of targets, but with Precision TALs you can target any locus in the genome, and this increase in engineering accuracy allows you to develop better solutions for healthcare, agriculture, energy, and bioproduction research.
Now every gene locus is within your reach
GeneArt® Precision TALs are supplied as Gateway® compatible entry clones encoding a DNA-binding protein specific to a customer-submitted sequence, fused to a range of customer-designated effector domains for genome editing. GeneArt® Precision TALs deliver outstanding performance:
How to order
If you have questions or need additional information about this service, please fill in our information request form.
GeneArt® Precision TALs are genome engineering tools for locus-specific modification of the genome
Precision TALs are produced in Xanthamonas, a genus of plant pathogens. The pathogen specifically recognizes DNA of the host with a protein containing 17–18 repeats of approximately 34 amino acids each. The nucleotide specificity is conferred by amino acids 12 and 13 within each repeat. Each nucleotide in the target DNA is recognized by one repeat. This 1-to-1 relationship can be used to specifically target any locus in the genome.
|Double-stranded breaks can be created at a customer-specified locus in the genome, using a pair of Precision TALs fused to Fok1 nuclease (Figure 1). Using a pair of Precision TALs for the targeting reduces off-target effects. Double-strand breaks are repaired through two mechanisms, homologous recombination or nonhomologous end joining. This repair process is used to perform targeting or gene silencing at any locus.|
|Gene activation is accomplished by specifically targeting a transcription activator to the gene of interest (Figure 2). The activator approach to gene over-expression has the advantage of expressing all the endogenous splice variants in the appropriate ratios.|
|Gene repression is accomplished by specifically targeting a transcription repressor to the gene of interest. (Figure 3).GeneArt® Precision TALs can be designed to act as repressors that will down-regulate the targeted gene, similar to the function of small interfering RNA (siRNA). Both gene activation and repression can serve to elucidate the role of the gene of interest in specific signaling pathways or other phenotypes.|
|If you want to deploy an effector domain not currently available, we offer a MCS (multiple cloning site) vector that allows you to insert any protein-coding sequence of interest. The resulting TAL will deliver that effector in a sequence-specific manner anywhere in the genome (Figure 4). Additional gene synthesis services are available to generate any effector domains for which you don’t have a template.|
Applications & Examples
In vitro cleavage of genomic target locus
|Surveyor Assay for in vivo modification
+ = Surveyor treated PCR product
- = untreated PCR product
Control TAL treated cells showed no modification
IL2RG TAL treated cells showed 7% modification
|Product Name||TAL||Effector Domain|
|Native TAL FokI||Native||Fokl|
|Truncated TAL FokI||Truncated||FokI|
|Native TAL vp16 activator||Native||vp16 activator|
|Native TAL vp64 activator||Native||vp64 activator|
|Native TAL MCS||Native||MCS|
|Truncated TAL MCS||Truncated||MCS|
- Boch J, et al. (2009) Breaking the code of DNA binding specificity of TAL-type III effectors. Science Dec 11;326(5959):1509-12.
- Moscou M, et al. (2009) A simple cipher governs DNA recognition by TAL effectors Science, Dec 11;326(5959):1501
- Miller J, et al. (2010) A TALE nuclease architecture for efficient genome editing. Nat. Biotech. Online pub.
- Geibler R, et al. (2011) Transcriptional Activators of Human Genes with Programmable DNA-Specificity. PLoS ONE 6(5): e19509.
- Hockemeyer D, et al. Genetic engineering of human pluripotent cells using TALE nucleases. (2011) Nat. Biotech. Online pub
For Research Use Only. Not for use in diagnostic procedures.