Obtain superior sensitivity and reproducibility without compromising any performance parameters in your real-time, quantitative PCR experiments.

  • Highly sensitive DNA quantitation to enable low copy number detection
  • Optimized formulation to offer improved consistency
  • Compatible with existing Applied Biosystems™ SYBR™ Green reagent protocols

Detect as few as 2 copies of a target gene

Applied Biosystems™ Power SYBR™ Green PCR Master Mix delivers highly sensitive nucleic acid quantitation, detecting as few as 2 copies of a target gene over a broad range of template concentrations. The newly optimized formulation contains highly purified Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD to offer greater sensitivity. Additionally, our new formulation minimizes variation, ensuring consistency between experiments. Most importantly, Power SYBR Green reagent–based PCR chemistry easily replaces Applied Biosystems™ SYBR™ Green PCR Master Mix in Applied Biosystems protocols using the same set up and thermal cycling conditions.

Sensitivity without compromise

Power SYBR Green PCR Master Mix delivers superior sensitivity and reproducibility without compromising any other performance parameters such as specificity, wide dynamic range and uniformity. In addition, Power SYBR Green PCR Master Mix provides high fluorescent signal strength for consistent detection of low copy numbers.

High precision

Power SYBR Green PCR Master Mix contains a proprietary version of Applied Biosystems™ ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations. Normalizing with an internal passive reference minimizes well-to-well variability that results from a variety of causes, such as pipetting error and sample evaporation.

Ready to use

Power SYBR Green PCR Master Mix provides greater flexibility, reduces experimental design time and requires fewer reagents. Power SYBR Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR Green reagent-based real-time PCR. Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD is a hot start PCR enzyme provided in an inactive state allowing for room temperature set up and minimizing non-specific amplification.

 

Product literature

Design and Optimization of SYBR Green Assays
Protocol for Comparing SYBR Green Master Mixes
Protocol: Power SYBR Green PCR Master Mix and RT-PCR
Product Bulletin: Power SYBR Green PCR Master Mix
Data Sheet: SYBR Green Master Mixes on LC 480
Data Sheet: SYBR Green Master Mixes on Bio-Rad CFX96

Data Sheet: SYBR Green Master Mixes on Bio-Rad iQ5
Quick Reference Card: Power SYBR Green PCR Master Mix: Performing Real-Time PCR Assays
Quick Reference Card: Power SYBR Green PCR Master Mix on iQ5
Quick Reference Card: Power SYBR Green PCR Master Mix LC 480
Quick Reference Card: Power SYBR Green PCR Master Mix on CFX96