Mini Gel Tank

One tank, 181 gels

The original Invitrogen Bolt Mini Gel Tank just got better—introducing the improved Invitrogen Mini Gel Tank for your convenience. Enhancements in design allow for increased usability so you can run a variety of different gel types in this unique tank design.

Compatible with these Invitrogen products:

Convenient and intuitive electrophoresis tank

  • Easy sample loading—with the new forward-facing well configuration
  • Less running buffer required—two separate gel chambers, just add buffer for each gel up to the marked fill line
  • Simultaneous visualization of both gels—streamlined, side-by-side tank configuration
  • Simplified monitoring of prestained protein markers—with new white tank stand
  • Versatile—compatible with Invitrogen NuPAGE, Bolt, or Novex mini gels
  • View Mini Gel Tank specifications

Mini Blot Module

Complete your Mini Gel Tank system with the innovative Mini Blot Module that conveniently fits into the chambers of the Mini Gel Tank and allows you to easily transfer proteins from mini gels to membranes.

Find compatible precast gels

See the NuPAGE mini gels, Novex mini gels, and Bolt gels selection guide
 

Resources for the Bolt Mini Gel Tank

Find FAQs, information on upgrading the original Bolt mini gel tank for versatility, and videos showing you how to use the Bolt mini gel tank with a variety of mini gels

View gel performance in the Mini Gel Tank

Equal performance of an Invitrogen Bolt 4–12% Bis-Tris Plus gel with protein standards and samples. The same protein standards and samples were loaded in 10 µL sample volumes in a new Bolt gel and an original Bolt gel. Similarly sharp, straight bands with nearly identical migration were observed with the new Bolt gel (A) as compared to the original Bolt gel (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 µg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 µg HeLa cell lysate; lane 5: 20 µg HeLa cell lysate; lane 6: 5 µg BSA; lane 7: 40 µg Jurkat cell lysate; lane 8: 5 µg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 µg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

Bolt gels

Equal performance of an Invitrogen NuPAGE Tris-Acetate gel with protein standards and samples when using a Mini Gel Tank or the XCell SureLock Mini-Cell Tank. The same protein standards and samples were loaded at 10 μL sample volumes in NuPAGE 3–8% Tris-Acetate gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with nearly identical migration were observed when using the Mini Gel Tank (A) as compared to the XCell SureLock Mini-Cell (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg BSA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

NuPAGE gels

Band quality with WedgeWell Tris-Glycine gels. Protein ladders, purified proteins and E. coli lysate were loaded on a 4–20% gradient Invitrogen WedgeWell Tris-Glycine gel. Straight lanes with good lysate protein band sharpness and resolution are observed. Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate (10 µL sample volume); lane 4: 6 µg BSA (10 µL sample volume); lane 7: 6 µg hIgG (10 µL sample volume); lane 8: 20 µg E. coli lysate (20 µL sample volume)

WedgeWell Tris-Glycine gel

Equal performance of am Invitrogen Novex Tricine gel with protein standards and samples when using a Mini Gel Tank or the XCell SureLock Mini-Cell Tank. The same protein standards and samples were loaded at 10 μL sample volumes in 10–20% Tricine gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with nearly identical migration were observed when using the Mini Gel Tank (A) as compared to the XCell SureLock Mini-Cell (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg BSA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

Novex Tricine gels

Equal performance of an Invitrogen Bolt 4–12% Bis-Tris Plus gel with protein standards and samples. The same protein standards and samples were loaded in 10 µL sample volumes in a new Bolt gel and an original Bolt gel. Similarly sharp, straight bands with nearly identical migration were observed with the new Bolt gel (A) as compared to the original Bolt gel (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 µg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 µg HeLa cell lysate; lane 5: 20 µg HeLa cell lysate; lane 6: 5 µg BSA; lane 7: 40 µg Jurkat cell lysate; lane 8: 5 µg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 µg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

Bolt gels

Equal performance of an Invitrogen NuPAGE Tris-Acetate gel with protein standards and samples when using a Mini Gel Tank or the XCell SureLock Mini-Cell Tank. The same protein standards and samples were loaded at 10 μL sample volumes in NuPAGE 3–8% Tris-Acetate gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with nearly identical migration were observed when using the Mini Gel Tank (A) as compared to the XCell SureLock Mini-Cell (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg BSA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

NuPAGE gels

Band quality with WedgeWell Tris-Glycine gels. Protein ladders, purified proteins and E. coli lysate were loaded on a 4–20% gradient Invitrogen WedgeWell Tris-Glycine gel. Straight lanes with good lysate protein band sharpness and resolution are observed. Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate (10 µL sample volume); lane 4: 6 µg BSA (10 µL sample volume); lane 7: 6 µg hIgG (10 µL sample volume); lane 8: 20 µg E. coli lysate (20 µL sample volume)

WedgeWell Tris-Glycine gel

Equal performance of am Invitrogen Novex Tricine gel with protein standards and samples when using a Mini Gel Tank or the XCell SureLock Mini-Cell Tank. The same protein standards and samples were loaded at 10 μL sample volumes in 10–20% Tricine gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with nearly identical migration were observed when using the Mini Gel Tank (A) as compared to the XCell SureLock Mini-Cell (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg BSA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

Novex Tricine gels

Mini Gel Tank specifications

Gel capacity Up to 2 mini gels 
Cell size 11 x 12 x 16 cm (height with lid on)
Buffer chamber Requirement 400 mL for each mini gel
Material Polycarbonate
Chemical resistance The Mini Gel Tank is impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.