Protein Gels

Explore our protein gel options. Choose from precast polyacrylamide gel electrophoresis (PAGE) chemistries designed for specific applications, each available in a variety of well and cassette formats, or select a system for pouring and casting your own gels.

Find the right gel for your protein

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Featured protein gel categories

  Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus Gels
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NuPAGE Bis-Tris Gels
NuPAGE Bis-Tris Gels
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Novex Tris-Glycine Gels
Novex Tris-Glycine Gels
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NuPAGE Tris-Acetate GelsNuPAGE Tris-Acetate Gels
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Tricine GelsNovex Tricine Gels
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Gel chemistry Bis-Tris Bis-Tris Tris-Glycine Tris-Acetate Tricine
Protein sample type Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) High range MW (40-500 kDa) Low range MW (2.5-40 kDa)
Product benefits Neutral pH for high sensitivity applications with larger loading capacity. Load up to 60 µL per well. Run times as fast as 20-35 minutes Neutral pH for high sensitivity applications such as western blotting, mass spectrometry Traditional Laemmli-style gels with larger loading capacity- load up to 60 µL per well Neutral pH for better separation, transfer and analysis of high molecular weight proteins Better separation, transfer and analysis of low molecular weight proteins
Recommened for Western blotting, mass spectrometry, post translationally modified proteins, dilute samples, denaturing applications Western blotting, mass spectrometry, post translationally modified proteins, denaturing applications Western blotting, in-gel staining, samples containing detergent and high salt, denaturing, or native PAGE applications High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, denaturing or native PAGE applications Low molecular weight proteins, western blotting, in-gel staining, denaturing applications

Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Bolt Bis-Tris Plus
  • Dilute or larger (up to 60 µL) samples 
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 6 to 400 kDa
  • Mini with larger well capacity (WedgeWell, up to 60 µL)
22–45 min up to 16 months
NuPAGE Bis-Tris
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 1.5 to 300 kDa
  • Mini
  • Midi
35–50 min 12 months

Tris-Glycine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

Novex Tris-Glycine

 

  • Separation of small- to medium-sized proteins using traditional Laemmli-style gels

6%, 8%, 10%, 12%,

4–20%, 4–12%, 14%,

16%, 8–16% , 10–20%

8 to 250 kDa

  • Mini with larger well capacity (WedgeWell, up to 60 µL)
  • Midi

90 min

up to 12 months

Tris-Acetate

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
NuPAGE Tris-Acetate
  • Separation of larger proteins
7%, 3–8% 36 to 500 kDa
  • Mini
  • Midi
60 min 6 months

Tricine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Novex Tricine
  • Separation of small proteins and peptides
10%, 16% 2 to 200 kDa
  • Mini
90 min 1–2 months

Native Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

NativePAGE Bis-Tris

  • Separation of native proteins
3–12%, 4–16% 15 to 10,000 kDa
  • Mini
90–120 min 12 months

IEF

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
IEF
  • Separation of proteins based on isoelectric point
pH 3–7, pH 3–10 pI 3.5 to pI 8.5
  • Mini
150 min 2 months

Zymogram

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Zymogram
  • Separation of proteins based on size and activity
10% (with gelatin) 10 to 220 kDa
  • Mini
90 min 2 months

E-PAGE

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
E-PAGE
  • High throughput for recombination product analysis and protein profiling
E-PAGE 96:6%
E-PAGE 48:8%
10 to 220 kDa
  • 48-well
  • 96-well
14–25 min 6 months
  NativePAGE Bis-Tris Gels
NativePAGE Bis-Tris Gels
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Isoelectric Focusing Gels
Isoelectric Focusing Gels
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Novex Zymogram Gels
Novex Zymogram Gels
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E-PAGE Precast Gels
E-PAGE Precast
Gels

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Gel chemistry Bis-Tris pH 3–7, pH 3–10 Tris-glycine with gelatin 8% or 6%
Protein sample type 15 to 10,000 kDa pI 3.5 to pI 8.5 10 to 220 kDa 10-200 kDa
Product benefits Separation of native proteins, neutral pH for high sensitivity applications Separation of proteins based on isoelectric point Separation of proteins based on size and activity High-throughput gel system, that eliminates the need for buffers
  SureCast Handcast System
SureCast Handcast System
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Empty Gel Cassettes
Empty Gel Cassettes
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Bolt Empty Cassettes
Bolt Empty Cassettes
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Cassette type Resuable, glass Single use, pre-sealed Single use, pre-sealed
Product benefits
  • Leak-free design—minimize the number of failed gels, waste less time
  • Superior glass plate durability
  • Unique tilt feature—minimize spillage and confidently load acrylamide solutions
  • Simple component assembly—using a single-motion load-and-lock mechanism
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
  • High capacity sample wells- load up to 60 µL per well
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
Gel dimensions Mini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8X13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)
Gel tank Mini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-Cell
Reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents
Combos available 1, 2, 10, 12, 15-wells 1, 2, 5, 10, 12, 15 and 2D-wells 10 or 12-wells (high capacity wells)
  Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus Gels
Explore
NuPAGE Bis-Tris Gels
NuPAGE Bis-Tris Gels
Explore
Novex Tris-Glycine Gels
Novex Tris-Glycine Gels
Explore
NuPAGE Tris-Acetate GelsNuPAGE Tris-Acetate Gels
Explore
Tricine GelsNovex Tricine Gels
Explore
Gel chemistry Bis-Tris Bis-Tris Tris-Glycine Tris-Acetate Tricine
Protein sample type Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) High range MW (40-500 kDa) Low range MW (2.5-40 kDa)
Product benefits Neutral pH for high sensitivity applications with larger loading capacity. Load up to 60 µL per well. Run times as fast as 20-35 minutes Neutral pH for high sensitivity applications such as western blotting, mass spectrometry Traditional Laemmli-style gels with larger loading capacity- load up to 60 µL per well Neutral pH for better separation, transfer and analysis of high molecular weight proteins Better separation, transfer and analysis of low molecular weight proteins
Recommened for Western blotting, mass spectrometry, post translationally modified proteins, dilute samples, denaturing applications Western blotting, mass spectrometry, post translationally modified proteins, denaturing applications Western blotting, in-gel staining, samples containing detergent and high salt, denaturing, or native PAGE applications High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, denaturing or native PAGE applications Low molecular weight proteins, western blotting, in-gel staining, denaturing applications

Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Bolt Bis-Tris Plus
  • Dilute or larger (up to 60 µL) samples 
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 6 to 400 kDa
  • Mini with larger well capacity (WedgeWell, up to 60 µL)
22–45 min up to 16 months
NuPAGE Bis-Tris
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 1.5 to 300 kDa
  • Mini
  • Midi
35–50 min 12 months

Tris-Glycine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

Novex Tris-Glycine

 

  • Separation of small- to medium-sized proteins using traditional Laemmli-style gels

6%, 8%, 10%, 12%,

4–20%, 4–12%, 14%,

16%, 8–16% , 10–20%

8 to 250 kDa

  • Mini with larger well capacity (WedgeWell, up to 60 µL)
  • Midi

90 min

up to 12 months

Tris-Acetate

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
NuPAGE Tris-Acetate
  • Separation of larger proteins
7%, 3–8% 36 to 500 kDa
  • Mini
  • Midi
60 min 6 months

Tricine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Novex Tricine
  • Separation of small proteins and peptides
10%, 16% 2 to 200 kDa
  • Mini
90 min 1–2 months

Native Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

NativePAGE Bis-Tris

  • Separation of native proteins
3–12%, 4–16% 15 to 10,000 kDa
  • Mini
90–120 min 12 months

IEF

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
IEF
  • Separation of proteins based on isoelectric point
pH 3–7, pH 3–10 pI 3.5 to pI 8.5
  • Mini
150 min 2 months

Zymogram

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Zymogram
  • Separation of proteins based on size and activity
10% (with gelatin) 10 to 220 kDa
  • Mini
90 min 2 months

E-PAGE

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
E-PAGE
  • High throughput for recombination product analysis and protein profiling
E-PAGE 96:6%
E-PAGE 48:8%
10 to 220 kDa
  • 48-well
  • 96-well
14–25 min 6 months
  NativePAGE Bis-Tris Gels
NativePAGE Bis-Tris Gels
View all
Isoelectric Focusing Gels
Isoelectric Focusing Gels
View all
Novex Zymogram Gels
Novex Zymogram Gels
View all
E-PAGE Precast Gels
E-PAGE Precast
Gels

View all
Gel chemistry Bis-Tris pH 3–7, pH 3–10 Tris-glycine with gelatin 8% or 6%
Protein sample type 15 to 10,000 kDa pI 3.5 to pI 8.5 10 to 220 kDa 10-200 kDa
Product benefits Separation of native proteins, neutral pH for high sensitivity applications Separation of proteins based on isoelectric point Separation of proteins based on size and activity High-throughput gel system, that eliminates the need for buffers
  SureCast Handcast System
SureCast Handcast System
Explore
Empty Gel Cassettes
Empty Gel Cassettes
Explore
Bolt Empty Cassettes
Bolt Empty Cassettes
Explore
Cassette type Resuable, glass Single use, pre-sealed Single use, pre-sealed
Product benefits
  • Leak-free design—minimize the number of failed gels, waste less time
  • Superior glass plate durability
  • Unique tilt feature—minimize spillage and confidently load acrylamide solutions
  • Simple component assembly—using a single-motion load-and-lock mechanism
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
  • High capacity sample wells- load up to 60 µL per well
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
Gel dimensions Mini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8X13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)
Gel tank Mini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-Cell
Reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents
Combos available 1, 2, 10, 12, 15-wells 1, 2, 5, 10, 12, 15 and 2D-wells 10 or 12-wells (high capacity wells)
Need help determining the best gel to use?
Protein Gels Performance Guarantee

Protein Gels Performance Guarantee

We stand behind the performance of our protein gels.

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Find the right protein gel for your experiments

Pour your own gels with a handcast system Value gels for screening and qualitative protein separation Gels based on traditional Laemmli formula with 60uL sample volume capacity Highest resolution, longest shelf-life and western optimized gels Highest resolution and long shelf-life
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  photo of gel cassette
View all handcast systems

Novex™ Value  Tris-Glycine gels
photo of Novex gel product
Novex™ WedgeWell™ Tris-Glycine gels
photo of Bolt gel product
Bolt™ Gels
photo of NuPAGE gel product
NuPAGE™ Gels

Citations in the last 10 years N/A N/A N/A N/A (new product) >20,000
Protein resolution & integrity, lot consistency & band quality Not applicable Good High High High
Average run time 90 min 46 min (225V) 60 min 35 min 35 min
Well format Standard Standard New high-capacity wedge design New high-capacity wedge design Standard
Gel dimensions Mini: 8x8 cm, 1 or 1.5 mm thick Mini: 8x8 cm, 1 mm thick Mini: 8x8 cm, 1 or 1.5 mm thick  Mini: 8x8.3 cm, 1 mm thick Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick
Shelf life N/A Up to 12 months Up to 12 months >16 months 8 months (Tris-Acetate) 16 months (Bis-Tris)
Pack sizes N/A 20 pack 2 & 10 packs 10 packs 2 & 10 packs