Reducing agents for protein disulfides

These purified powders, convenient solutions, and solid-phase resins of disulfide reducing agents, including DTT, BME and TCEP, can be used to stabilize free sulfhydryls (cysteines) and to reduce disulfide bonds in peptides and proteins.

Chaotropic and denaturing chemical agents, including urea and guanidine hydrochloride, disrupt water interactions and promote hydrophobic protein and peptide solubilization, elution, refolding, and structural analysis.

Choose the right disulfide reducing agent or denaturant for your experiments

Order products Function
2-Mercaptoethanol Pure liquid (14 M), beta-mercaptoethanol (BME, 2BME, 2-ME, b-mer, CAS 60-24-2) is a thiol reducing agent for cleaving protein disulfide bonds (cystine).
2-Mercaptoethylamine-HCl
Pure crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl, CAS 156-57-0), selectively reduces antibody hinge-region disulfide bonds.
Bond-Breaker TCEP Solution, Neutral pH
Stable 0.5 M solution of TCEP reducing agent for protein disulfide bonds, odor-free and suitable as a 10X stock to make reducing SDS-PAGE sample buffers.
Cysteine-HCl Pure crystalline cysteine hydrochloride (Cys-HCl, CAS 52-89-1) is used to make the sulfhydryl assay standards or as an additive in protein refolding experiments.
Dithiothreitol
Pure crystalline dithiothreitol (DTT, CAS 3483-12-3) is a popular protein disulfide reducing agent for sample loading buffers.
TCEP-HCl
Pure crystalline Tris (2-carboxyethyl) phosphine hydrochloride, (TCEP, CAS 5961-85-3) is a thiol-free reductant for protein and peptide disulfide bonds.
TCEP-HCl, Premium Grade
Highly characterized version of a popular protein disulfide reducing agent thoroughly tested and fully documented.
Immobilized Reductant Columns
Thermo Scientific Pierce immobilized reductant column contains resin to which a thiol-based reducing agent has been immobilized to enable the solid-phase reduction of peptide and protein disulfide bonds.
Immobilized TCEP Disulfide Reducing Gel
Immobilized TCEP disulfide reducing gel is tris(2-carboxyethyl)phosphine covalently immobilized to agarose resin to help enable the efficient reduction of peptide and protein disulfide bonds without contaminating samples with reducing agent.
8M Guanidine-HCl Solution Accurately formulated, highly pure, ready-to-use solution for the solubilization of hydrophobic proteins and peptides.
Guanidine-HCl Highly pure solid that is easily dissolved and pH-adjusted for the solubilization of hydrophobic proteins and peptides.
Urea Pure, crystalline protein denaturant that increases solubility of hydrophobic molecules and acts as a general protein denaturant via unfolding and altering their 3D structures.

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