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Activity data for the EmrE membrane protein expressed using the MembraneMax™ kit
Activity assays showed that the EmrE is active only when MembraneMax™ reagent is included (+) in the expression reaction (inset). The substrate [3h]tetraphenylphosphonim (TPP+) was used as ligand. Binding constants for purified EmrE complexed with his-NLPs were in agreement with a previous report indicating that EmrE is correctly folded, and therefore correctly inserted into the lipid bilayer of the MembraneMax™ reagent.
![Activity assays showed that the EmrE is active only when MembraneMax™ reagent is included (+) in the expression reaction (inset). The substrate [3h]tetraphenylphosphonim (TPP+) was used as ligand. Binding constants for purified EmrE complexed with his-NLPs were in agreement with a previous report indicating that EmrE is correctly folded, and therefore correctly inserted into the lipid bilayer of the MembraneMax™ reagent.](http://tools.thermofisher.com/content/sfs/gallery/low/membranemax-4.jpg)
