Veterinary Drug Residues Testing Information

Innovative technologies to enable multi-residue detection

Antibiotics are a group of chemicals that are widely used in animal husbandry primarily for protection of animals from disease but also as growth promoters. The European Union (EU) has set maximum residue limits (MRL) for a variety of antibiotics in animal tissues, milk and eggs; suitable methods are required to be capable of detecting these residues at regulated levels.  For quantitative analysis it is necessary to use instrumental techniques such as LC-MS/MS. This technique can also be used for screening, and provides much higher sensitivity and greater specificity. 


Screening Method for Fluoroquinolones Using an Advanced UHPLC Column and System Combination

Fluoroquinolones are poly-aromatic compounds with fluorine atoms and carboxylic acid functionality. They are sensitive to silanol interactions with the stationary phase that can lead to compromised peak shape and reduce effective peak resolution.

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System

To describe a new method for veterinary drug analysis, showing how the variable data-independent acquisition (vDIA) workflow achieves high sensitivity and selectivity, providing a complete high-quality data record of the measured sample.

Featured video

In a NACRW 2015 interview, Ed George discusses single methodology for the analysis of multi-class veterinary drugs in different animal matrices used for consumption.


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Type Title Category Resource #
Application Note Determination of Virginiamycin, Erythromycin, and Penicillin in Dried Distillers Grains with Solubles Liquid Chromatography AN1055
Application Note Trace Level Determination of Eleven Antiprotozoal Agents in Eggs after Simple Matrix Clean-Up LC-MS AN116
Poster Note Online Sample Preparation for the Quantitative Screening of Multiple Veterinary Drug Residues in Chicken, Beef and Pork LC-MS  
Application Note Simple and Rapid Analysis of Chloramphenicol in Milk by LC-MS/MS LC-MS AN407
Application Note Quantitative Analysis of Ractopamine in Beef using Automated Online Sample Preparation with Liquid Chromatography-Tandem Mass Spectrometry LC-MS AN63554
Application Note Screening Method for Fluoroquinolones Using an Advanced UHPLC Column and System Combination UHPLC AN21500
Application Note Multi-class Antibiotic Screening of Honey Using Online Extraction with LC-MS/MS LC-MS AN464
Application Note Measurement of Chloramphenicol in Honey Using Automated Sample Preparation with LC-MS/MS LC-MS AN473
Application Note LC-MS/MS Determination of Malachite Green and Leucomalachite Green in Fish Products LC-MS AN385
Application Note LC-MS/MS Analysis of Malachite Green, Leucomalachite Green, Ciprofloxacin, and Tetracycline in Food Samples using a TurboFlow Method LC-MS AN442
Application Note Study of ß-agonist Residues in Animal-derived Foods by LC-MS/MS LC-MS AN481
Application Note Determination of Trace Level Nitrofuran Metabolites in Crawfish Meat by Electrospray LC-MS/MS on the TSQ Quantum Discovery MAX LC-MS AN361
Application Note Detection of Glucocorticoid Residues in Animal Derived Food by HPLC-MS/MS LC-MS AN482
Technical Guide Validated Method for the Determination of Phenylbutazone in Horse Meat with LC-MS/MS LC-MS TG63799
Poster Note Determination of Veterinary Drug Residues in Milk by GC/MS and GC/ECD GC-MS PN10390
Poster Note A New Approach to the Simultaneous Analysis of Underivatized Ionophoric Antibiotics using Liquid Chromatography with Charged Aerosol Detection Liquid Chromatography PN70054
Poster Note Analysis of Multiclass Veterinary Drugs in Baby Food by Ultra Fast Chromatography with High Performance Triple Quadrupole LC-MS PN-ASMS13
Technical Guide Multi-residue Automated Turbulent Flow Online LC-MS/MS Method for the Determination of Antibiotics in Milk LC-MS TG63551
Technical Guide Method Automated Online Multi-Residue LC-MS/MS Method for the Determination of Antibiotics in Chicken Meat LC-MS TG63646
Technical Note Determination of Clenbuterol from Bovine Urine Samples using Multiskan FC Immunoassay AN-MR-MSFC11-1110
Poster Note vDIA Approach Non-Target-Screening Vet Drugs in Animal Feed Liquid Chromatography PN64426

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