フローサイトメトリー用の細胞周期アッセイ

フローサイトメトリーは、モデリングアルゴリズムと組み合わせると、G0/G1 期、S 期、G2 期の細胞周期もしくは倍数性を評価する強力なツールとなります。Molecular Probes® は、生細胞でも固定細胞でも正確に細胞周期を解析するため、さまざまな蛍光色素を提供しています。

Vybrant® DyeCycle™ 試薬

Vybrant® DyeCycle™色素シリーズは生細胞の細胞周期解析用の蛍光色素であり細胞毒性が低く、405、488、532、または633 nmの励起光を使用します。色素は低細胞毒性で、染色された細胞をソーティングしたり、あるいは染色後に培養または機能アッセイで評価することができます。

FxCycle™ Violet、SYTOX® AADvanced™、FxCycle™ Far Red試薬を使用して細胞周期を解析します。複数の色素が選択できるため、固定細胞の細胞周期解析が簡単になります。

	Hoechst 33342	Vybrant® DyeCycle™ Violet	Vybrant® DyeCycle™ Green	Vybrant® DyeCycle™ Orange	Vybrant® DyeCycle™ Ruby
Basis of assay	Utilize fluorescent dyes to distinguish cells in G0/G1 phase versus S phase, G2, or polyploidy
Readout	DNA content analysis in living cells using a is cell permeable dye, which after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass
Excitation	UV or 350	UV or 405	488	488 or 532	488 or 633/5
Emission	461	437	534	563	686
Recommended filter	450/50	440/40	530/30	585/42	695/40
Multiplexable
Sample type	The dyes have low cytotoxicity, allowing stained cells to be sorted and otherwise cultured or assessed with functional assays after staining.
Format	10 mL	200 assays	200 assays	200 assays	100 assays	400 assays
Cat. No.	H3570	V35003	V35004	V53005	V10309	V10273
Bibliography	Citations

	Propidium Iodide ReadyProbes® Reagent	FxCycle™ Violet	FxCycle™ PI/RNAse Solution	FxCycle™ Far Red	SYTOX® AADvanced™
Basis of assay	Utilize fluorescent dyes to distinguish cells in G0/G1 phase versus S phase, G2, or polyploidy
Readout	DNA content analysis using a cell permeable dye, which after binding double-stranded DNA when cells are alive, emits a fluorescent signal that is proportional to the DNA mass even after fixation.
Excitation	488 or 532	405	488 or 532	633	488 or 532
Emission	617	358/461	617	660/20	695/40
Recommended filter	575/24	450/50	572/24	660/20	695/40
Multiplexable
RNase required			Included
Sample type	These stains have been designed for flow cytometric analysis of DNA content in fixed cells.
Format	1 kit	1 kit	100 mL	1 kit	5 x 0.5 mL	0.2 mL
Cat. No.	R37108	F10347	F10797	F10348	S10274	S10349

Poster	Presented	Year
Non-cytotoxic Near-IR DNA Stain for Cell Cycle Analysis in Living Cells	CYTO	2010
Dual Pulse Labeling of S-Phase Population Using Click Chemistry to Measure Changes In Cell Proliferation	ASCB	2009
Dual Pulse Labeling Using a New Thymidine analog 5-Ethynyl-2’-Deoxyuridine (Edu) to Detect alterations In S-Phase Progression by Fluorescence Microscopy and Flow Cytometry	ASCB	2009
Detection of S-Phase Cell Cycle Progression Using 5-Ethynyl-2’-Deoxyuridine Incorporation With Click Chemistry	Probe Discovery	2008
Characterization of DNA Content, Cyclin B1 and Phosphorylated Histone H3 With Direct S-Phase Using Edu Incorporation In Multiparameter Testing of Cell Lines With Cell Cycle Blocking Agents	ISAC	2008
Detection of Dna Synthesis by automated Microscopy and Image analysis: Comparison of Brdu Method and a New Click Chemistry Based Edu Method	ISAC	2008
Effects of The Thymidine analogues Edu and Brdu On Cell Viability and Cycle Progression	ISAC	2008
Evaluation of Click Chemistry Based Alternative to BrdU Antibody Labeling in Tissue and Cultured Cells Using Fluorescence Microscopy and Flow Cytometry	ASCB	2007

Resources

For Research Use Only. Not for use in diagnostic procedures.