ProcartaPlex Assays
Profile more biomarkers with less sample
Invitrogen ProcartaPlex multiplex immunoassays use the Luminex xMAP (multianalyte profiling) technology that enables the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. The technology uses differentially dyed capture beads for each target in a multiplex “ELISA-like” assay. ProcartaPlex immunoassays can profile up to 80 times more analytes using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
ProcartaPlex multiplex immunoassays provide the ability to create a unique panel with the assurance to achieve reproducible results throughout the course of your study.
Features of ProcartaPlex assays
- Magnetic bead format for increased ease-of-use and throughput
- More than 90% of ProcartaPlex assays can be combined with one another
- High correlation to our ELISA assays (R2 >0.9)
- Scalable and reproducible performance regardless of plex size
- Largest published panel for quantitation of up to 65 analytes
ProcartaPlex multiplex panels
Multiplex panels are pre-configured biologically-defined and disease-defined panels using magnetic beads for quantitative multiplex analysis of multiple targets in a single sample. ProcartaPlex immunoassays are available in multiple formats across six species (human, mouse, rat, non-human primate, porcine, and canine).
View full listing of multiplex panels
Panel features
- Choose from a variety of commonly investigated biologically- and disease-defined analyte panels.
- Quantitatively measure secreted biomarkers in plasma, serum, other bodily fluids, and cell culture supernatants.
- Select “convenience” panels to use the assay as configured, or select “configurable” panels to customize the assay by combining with another panel or adding compatible ProcartaPlex simplex beads.
- Alternatively, simplex kits can be purchased individually with a ProcartaPlex basic Kit (includes all non-analyte specific reagents needed to run the ProcartaPlex assay) to create your unique customized multiplex panel.
- Selected panels are available in "Platinum" or "High-Sensitivity" formats (see sections that follow).
Human combinable panels
Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.
| Human Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine/Chemokine/Growth Factor 45-Plex Human Panel 1 | EPX450-12171-901 | BDNF, EGF, Eotaxin, FGF-basic, GM-CSF, GROα, HGF, IFNγ, IFNα, IL-1RA, IL-1β, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1α, MIP-1β, NGFβ, PDGF-BB, PLGF, RANTES, SCF, SDF1α, TNFα, TNFβ, VEGF-A, VEGF-D |
| Cytokine & Chemokine 34-Plex Human Panel 1A | EPX340-12167-901 | Eotaxin, GM-CSF, GROα, IFNα, IFNγ, IL-1β, IL-1α, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFα, TNFβ |
| Cytokine 25-Plex Human Panel 1B | EPX250-12166-901 | GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, TNFα, TNFβ |
| Inflammation 20-Plex Human Panel | EPX200-12185-901 | E-Selectin, GM-CSF, ICAM-1, IFNα, IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IP-10, MCP-1, MIP-1α, MIP-1β, P-Selectin, TNFα |
| Th1/Th2 & Chemokine 20-Plex Human Panel 1 | EPX200-12173-901 | Eotaxin, GM-CSF, GROα, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-12 p70, IL-13, IL-18, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFα |
| Th1/Th2 & Cytokine 18-Plex Human Panel 1C | EPX180-12172-901 | GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-12 p70, IL-13, IL-15, IL-18, IL-31, TNFα, TNFβ |
| Th1/Th2/Th9/Th17/Th22/Treg Cytokine 18-Plex Human Panel | EPX180-12165-901 | GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, TNFα |
| Th9/Th17/Th22/Treg & Chemokine 16-Plex Human Panel 1 | EPX160-12175-901 | Eotaxin, GROα, IL-8, IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27,IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α |
| Cytokine 1C & Chemokine 16-Plex Human Panel 1 | EPX160-12176-901 | Eotaxin, GROα, IFNα, IL-1α, IL-1RA, IL-7, IL-8, IL-15, IL-31, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFβ |
| Th9/Th17/Th22/Treg & Cytokine 14-Plex Human Panel 1C | EPX140-12174-901 | Eotaxin, GROα, IL-8, IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27,IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α |
| Immuno-Oncology Checkpoint 14-Plex Human Panel | EPX14A-15803-901 | BTLA, CD28, CD80, CD137, CD27, CD152, GITR, HVEM, IDO, LAG-3, PD-1, PD-L1, PD-L2, TIM-3 |
| Growth Factor 11-Plex Human Panel | EPX110-12170-901 | BDNF,NGFβ, EGF, FGF-basic, HGF, LIF, PDGF-BB, PlGF, SCF, VEGF-A, VEGF-D |
| Th1/Th2 Cytokine 11-Plex Human Panel | EPX110-10810-901 | GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12 p70, IL-13, IL-18, TNFα |
| Chemokine 9-Plex Human Panel 1 | EPX090-12187-901 | Eotaxin, GROα, RANTES, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, SDF1α |
| Th9/Th17/Th22/Treg Cytokine 7-Plex Human Panel | EPX070-10817-901 | IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27 |
| Cytokine 7-Plex Human Panel 1C | EPX070-10010-901 | IFNα, IL-1α, IL-1RA, IL-7, IL-15, IL-31, TNFβ |
| Essential Th1/Th2 Cytokine 6-Plex Human Panel | EPX060-10009-901 | IFNγ, IL-4, IL-5, IL-6, IL-12 p70, TNFα |
| MMP 5-Plex Human Panel 1 | EPX050-10015-901 | MMP-1, MMP-7, MMP-8, MMP-12, MMP-13 |
| Essential 4-Plex Human Panel 2 | EPX040-10008-901 | IL-8, IL-1β, IL-10, IL-17A |
| MMP 3-Plex Human Panel 2 | EPX030-10829-901 | MMP-2, MMP-3, MMP-9 |
Human convenience panels
Beads and detection antibodies are premixed in one vial each. Panels are not combinable with other ProcartaPlex assays.
| Human Panel Name | Cat. No. | Antigens |
|---|---|---|
| Immune Monitoring 65-Plex Human Panel | EPX650-10065-901 | APRIL, BAFF, BLC, CD30, CD40L, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, FGF-2, Fractalkine, G-CSF, GM-CSF, Gro a, HGF, IFN-a, IFN-g, IL-10, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-18, IL-1a,IL-1b, IL-2, IL-20, IL-21, IL-22, IL-23, IL-27, IL-2R, IL-3, IL-31, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IP-10, I-TAC, LIF, MCP-1, MCP-2, MCP-3, M-CSF, MDC, MIF, MIG, MIP1a, MIP-1b, MIP-3a, MMP-1,NGF beta, SCF, SDF-1a, TNF b, TNF-a, TNF-R2, TRAIL, TSLP, TWEAK, VEGF-A |
| Cytokine/Chemokine/Growth Factor Convenience 45-Plex Human Panel 1 | EPXR450-12171-901 | BDNF, EGF, Eotaxin, FGF-basic, GM-CSF, GROα, HGF, IFNγ, IFNα, IL-1RA, IL-1β, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1α, MIP-1β, NGFβ, PDGF-BB, PLGF, RANTES, SCF, SDF1α, TNFα, TNFβ, VEGF-A, VEGF-D |
| Cytokine & Chemokine Convenience 34-Plex Human Panel 1A | EPXR340-12167-901 | Eotaxin, GM-CSF, GROα, IFNα, IFNγ, IL-1β, IL-1α, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFα, TNFβ |
| TNF Superfamily 15-Plex Human Panel | EPX150-15801-901 | 4-1BBL, APRIL, BAFF, CD30L, EDA-1, FasL, GITRL, CD40L, NGFβ, TNFα, TNFβ, LIGHT, RANKL, TRAIL, TWEAK |
| TNF Receptor Superfamily 12-Plex Human Panel | EPX120-15802-901 | Fas (APO), CD27, CD30, CD40, 4-1BB , DR6, GITR, HVEM, TNF-R1, TNF-R2, TRAIL-R1,TRAIL-R2 |
| Metabolism/Obesity 9-Plex Human Panel 2 | EPX09A-15804-901 | C-peptide, Cortisol, FGF-23, GLP-1, Ghrelin, Insulin, PYY, Leptin, Resisitin |
| Graft Versus Host Disease 8-Plex Human Panel | EPX080-15814-901 | CD30, Granzyme A, HGF, IL-2R (CD25), KRT18, ST2/IL33R, Syndecan, TNF RI |
| Myokine 8-Plex Human Panel | EPX080-12186-901 | BDNF, IL-1RA, IL-10, IL-15, IL-6, IL-8, LIF, TNFα |
| Antibody Isotyping 7-Plex Human Panel | EPX070-10818-901 | IgG1, IgG2, IgG3, IgG4, IgE, IgA, IgM |
| Coagulation 6-Plex Human Panel 1 | EPX060-10824-901 | Factor XI, Factor XII, Factor XIII, Antithrombin, Prothrombin, CRP |
| Metabolism/Obesity 5-Plex Human Panel 1 | EPX050-15805-901 | Adiponectin, ICAM-1, NGAL, ZAG, RBP4 |
| Coagulation 4-Plex Human Panel 3 | EPX040-10825-901 | Factor IX, Protein S, Protein C, vWF |
| Coagulation 3-Plex Human Panel 2 | EPX030-10823-901 | Factor V, Factor VII, Factor VIII |
Mouse combinable panels
Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.
| Mouse Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine & Chemokine 36-Plex Mouse Panel 1A | EPX360-26092-901 | ENA-78, Eotaxin, G-CSF, GM-CSF, GROα, IFNα, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-15/IL-15R, IL-17A, IL-18, IL-22, IL-23, IL-27, IL-28, IL-31, IP-10, LIF, MCP-1, M-CSF, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα |
| Cytokine & Chemokine 26-Plex Mouse Panel 1 | EPX260-26088-901 | Eotaxin, GM-CSF, GROα, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα |
| Th1/Th2 & Chemokine 20-Plex Mouse Panel 1 | EPX200-26090-901 | Eotaxin, GROα, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12 p70, IL-13, IL-18, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα |
| Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse Panel | EPX170-26087-901 | GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, TNFα |
| Th9/Th17/Th22/Treg & Chemokine 15-Plex Mouse Panel | EPX150-26089-901 | IL-10, IL-17A, IL-22, IL-23, IL-27, IL-9, GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, Eotaxin |
| Th1/Th2 Cytokine 11-Plex Mouse Panel | EPX110-20820-901 | GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12 p70, IL-13, IL-18, TNFα |
| Cytokine 10-Plex Mouse Panel 1B | EPX100-26091-901 | ENA-78, G-CSF, IFNα, IL-1α, IL-3, IL-15/IL-15R, IL-28, IL-31, LIF, M-CSF |
| Chemokine 9-Plex Mouse Panel 1 | EPX090-20821-901 | GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, Eotaxin |
| Essential 8-Plex Mouse Panel 2 | EPX080-20832-901 | IL-1β, IL-10, IL-17A, GROα, MCP-1, MIP-2, MIP-1α, MIP-1β |
| Th9/Th17/Th22/Treg Cytokine 6-Plex Mouse Panel | EPX060-20822-901 | IL-9, IL-10, IL-17A, IL-22, IL-23, IL-27 |
| Essential Th1/Th2 Cytokine 6-Plex Mouse Panel | EPX060-20831-901 | IFNγ, IL-4, IL-5, IL-6, IL-12 p70, TNFα |
Mouse convenience panels
Beads and detection antibodies are premixed in one vial each. Panels are not combinable with other ProcartaPlex assays.
| Mouse Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine & Chemokine Convenience 36-Plex Mouse Panel 1A | EPXR360-26092-901 | ENA-78, Eotaxin, G-CSF, GM-CSF, GROα, IFNα, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-15/IL-15R, IL-17A, IL-18, IL-22, IL-23, IL-27, IL-28, IL-31, IP-10, LIF, MCP-1, M-CSF, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα |
| Cytokine & Chemokine Convenience 26-Plex Mouse Panel 1 | EPXR260-26088-901 | Eotaxin, GM-CSF, GROα, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα |
| Antibody Isotyping 7-Plex Mouse Panel 2 | EPX070-20816-901 | IgG1, IgG2b, IgG2c, IgG3, IgA, IgE, IgM |
| Antibody Isotyping 7-Plex Mouse Panel | EPX070-20815-901 | IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, IgM |
| Myokine 5-Plex Mouse Panel | EPX050-20833-901 | IL-10, IL-15/IL-15R, IL-6, LIF, TNFα |
| IFN alpha/IFN beta 2-Plex Mouse Panel | EPX020-22187-901 | IFNα, IFN β |
| Canine Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine/Chemokine/Growth Factor 11-Plex Canine Panel 1 | EPX11A-50511-901 | IFNγ, IL-2, IL-6, IL-8, IL-10, IL-12 p40, MCP-1, NGFβ, SCF, TNFα, VEGF-A |
| Porcine Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine & Chemokine 9-Plex Porcine Panel 1 | EPX090-60829-901 | IL-1 β, IL-4, IL-6, IL-8, IL-10, IL-12p40, IFNα, IFNγ, TNFα |
Rat combinable panels
Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.
| Rat Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine & Chemokine 22-Plex Rat Panel | EPX220-30122-901 | IL-1α, G-CSF, IL-10, IL-17A, IL-1β, IL-6, TNFα, IL-4, GM-CSF, IFNγ, IL-2, IL-5, IL-13, IL-12p70, Eotaxin, GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-2, RANTES |
| Th Complete 14-Plex Rat Panel | EPX140-30120-901 | L-1 α, IL-1 β, GM-CSF, G-CSF, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A/CTLA-8, TNFα |
| Chemokine 8-Plex Rat Panel | EPX080-30121-901 | GROα, MCP-1, MIP-1α, RANTES, Eotaxin, IP-10, MCP-3, MIP-2 |
Rat convenience panels
Beads and detection antibodies are premixed in one vial each. Panels are not combinable with other ProcartaPlex Assays.
| Rat Panel Name | Cat. No. | Antigens |
|---|---|---|
| Antibody Isotyping 6-Plex Rat Panel | EPX060-30123-901 | IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM |
| Kidney Toxicity 5-Plex Rat Panel 1 | EPX050-30124-901 | KIM-1, osteopontin, TFF3, VEGF-A, calbindin |
| Kidney Toxicity 5-Plex Rat Panel 2 | EPX050-30125-901 | Albumin, clusterin, cystatin, NGAL, TIMP-1 |
Non-human primate combinable panels
Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.
| Non-Human Primate Panel Name | Cat. No. | Antigens |
|---|---|---|
| Cytokine/Chemokine/Growth Factor 37-Plex NHP Panel | EPX370-40045-901 | BDNF, BLC, Eotaxin, FGF-2, G-CSF, GM-CSF, IFNα, IFNγ, IL-1β, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-18, IL-1RA, IL-2, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, NGF β, PDGF-BB, sCD40L, SCF, SDF-1α, TNFα, VEGF-A, VEGF-D |
| Cytokine & Chemokine 30-Plex NHP Panel | EPX300-40044-901 | BLC, Eotaxin, G-CSF , GM-CSF, IFNα, IFNγ, IL-1β, IL-10, IL-12p70, IL-13, IL-15, IL-17A /CTLA-8, IL-18, IL-1RA, IL-2, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, sCD40L, SDF-1α, TNFα |
| Th 14-Plex NHP Panel | EPX140-40040-901 | IFNγ, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-5, IL-6, TNFα, GM-CSF, IL-18, IL-10, IL-17A, IL-23 |
| Chemokine 10-Plex NHP Panel | EPX100-40041-901 | IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, Eotaxin, SDF-1α, BLC, I-TAC, MIG |
| Growth Factor 7-Plex NHP Panel | EPX070-40043-901 | BDNF, FGF-basic, PDGF-BB, SCF, VEGF-A, VEGF-D, NGFβ |
| Cytokine 6-Plex NHP Panel 1B | EPX060-40042-901 | G-CSF, IFNα, IL-1RA, IL-15, IL-7, CD40L |
Cross-reactivity chart for NHP ProcartaPlex simplex kits
| Cat. No. | Analyte | Chim- panzee |
Afr. gr. monkey |
Ba-boon | Cyno- molgus |
Pigtail macaque |
Rhesus monkey |
Mar- moset |
Squirrel monkey |
|---|---|---|---|---|---|---|---|---|---|
| EPX01A-42032-901 | Adiponectin | +++ | N.T. | + | + | N.T. | ++ | - | - |
| EPX01A-42116-901 | BDNF | +++ | ++ | + | + | + | ++ | + | + |
| EPX01A-42147-901 | BLC/CXCL13 | +++ | +++ | +++ | +++ | ++ | +++ | ++ | + |
| EPX01A-40239-901 | CD40L | +++ | + | +++ | - | - | +++ | +++ | + |
| EPX01A-42120-901 | Eotaxin | +++ | + | +++ | +++ | +++ | +++ | +++ | ++ |
| EPX01A-42074-901 | FGF-2 | +++ | +++ | ++ | +++ | ++ | +++ | +++ | +++ |
| EPX01A-42001-901 | G-CSF | +++ | + | +++ | +++ | +++ | +++ | ++ | + |
| EPX01A-40283-901 | GM-CSF | +++ | +++ | + | +++ | + | + | + | + |
| EPX01A-42027-901 | Granzyme B | +++ | - | - | - | - | - | - | - |
| EPX01A-40201-901 | ICAM-1 | +++ | +++ | +++ | +++ | +++ | +++ | ++ | ++ |
| EPX01A-40216-901 | IFN alpha | ++ | - | + | ++ | - | ++ | ++ | - |
| EPX01A-40228-901 | IFN gamma | +++ | +++ | +++ | +++ | ++ | +++ | +++ | +++ |
| EPX01A-40224-901 | IL-1 beta | +++ | + | +++ | +++ | ++ | +++ | ++ | ++ |
| EPX01A-40642-901 | IL-10 | +++ | + | + | + | - | + | ++ | + |
| EPX01A-42090-901 | IL-12p40 | +++ | +++ | +++ | +++ | ++ | +++ | +++ | +++ |
| EPX01A-40238-901 | IL-12p70 | +++ | ++ | ++ | + | + | + | +++ | +++ |
| EPX01A-40231-901 | IL-13 | +++ | + | +++ | + | +/- | ++ | +/- | +/- |
| EPX01A-42089-901 | IL-15 | +++ | ++ | ++ | ++ | + | +++ | +++ | + |
| EPX01A-42017-901 | IL-17A | +++ | +++ | ++ | ++ | ++ | +++ | +++ | + |
| EPX01A-42160-901 | IL-17F | +++ | ++ | ++ | ++ | ++ | ++ | + | + |
| EPX01A-40267-901 | IL-18 | +++ | +++ | ++ | ++ | ++ | ++ | ++ | + |
| EPX01A-42080-901 | IL-1RA | +++ | ++ | +++ | +++ | +++ | +++ | + | +/- |
| EPX01A-40221-901 | IL-2 | +++ | - | - | - | - | - | + | + |
| EPX01A-42023-901 | IL-23 | +++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ |
| EPX01A-40225-901 | IL-4 | +++ | +++ | +++ | +++ | ++ | +++ | ++ | ++ |
| EPX01A-40278-901 | IL-5 | +++ | ++ | +++ | ++ | + | ++ | ++ | - |
| EPX01A-40213-901 | IL-6 | +++ | + | ++ | +++ | +++ | +++ | +/- | +/- |
| EPX01A-40237-901 | IL-7 | +++ | - | ++ | ++ | - | +++ | +++ | + |
| EPX01A-40204-901 | IL-8 | +++ | ++ | +++ | +++ | +++ | +++ | +/- | +/- |
| EPX01A-40284-901 | IP-10 | +++ | +/- | +/- | +/- | +/- | +/- | +/- | +/- |
| EPX01A-42124-901 | I-TAC | +++ | +++ | +++ | +++ | ++ | +++ | ++ | ++ |
| EPX01B-40281-901 | MCP-1 | +++ | +++ | +++ | +++ | +++ | +++ | +/- | +/- |
| EPX01A-42125-901 | MCP-3 | + | + | + | + | + | + | +++ | + |
| EPX01A-42127-901 | MIF | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| EPX01A-40285-901 | MIG | +++ | + | ++ | +++ | ++ | +++ | ++ | ++ |
| EPX01A-42029-901 | MIP-1 alpha | +++ | +++ | +++ | +++ | ++ | +++ | ++ | +++ |
| EPX01A-42030-901 | MIP-1 beta | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| EPX01A-42117-901 | NGF beta | +++ | + | ++ | - | - | +++ | +++ | +++ |
| EPX01A-42033-901 | PAI-1 | +++ | ++ | + | + | + | +++ | +++ | ++ |
| EPX01A-42071-901 | PDGF-BB | +++ | ++ | +++ | +++ | +/- | +++ | +++ | +++ |
| EPX01A-40287-901 | RANTES | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| EPX01A-42040-901 | Resistin | +++ | +++ | +++ | ++ | +++ | + | +++ | +/- |
| EPX01A-42137-901 | SCF | + | N.T. | N.T. | N.T. | N.T. | + | ++ | + |
| EPX01A-42138-901 | SDF-1 alpha | +++ | +++ | +++ | +++ | +++ | +++ | + | +/- |
| EPX01A-42139-901 | TGF alpha | ++ | + | + | + | + | - | - | + |
| EPX01A-40249-901 | TGF beta 1 | +++ | +++ | ++ | ++ | ++ | +++ | +++ | ++ |
| EPX01A-40223-901 | TNF alpha | +++ | ++ | +++ | +++ | +++ | +++ | +++ | + |
| EPX01A-40202-901 | TNF beta | +++ | ++ | ++ | +++ | ++ | +++ | - | - |
| EPX01A-42004-901 | TRAIL | +++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ |
| EPX01A-40232-901 | VCAM-1 | +++ | +/- | +/- | - | +/- | - | - | - |
| EPX01A-40277-901 | VEGF A | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ++ |
| EPX01A-42076-901 | VEGF D | N.T. | N.T. | N.T. | N.T. | N.T. | N.T. | ++ | N.T. |
N.T. = Not tested
Simplex bead sets are magnetic bead sets used to detect individual analytes. They are designed to be added to ProcartaPlex panels to increase customization. Alternatively, multiple ProcartaPlex simplex bead sets can be combined and run using the ProcartaPlex Basic Kit that includes all non-target specific reagents needed to perform the ProcartaPlex assay.
View full listing of simplex kits
ProcartaPlex basic kits
| Cat. No. | Basic Kit | Description |
|---|---|---|
| EPX010-10420-901 | Human Basic Kit | Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay |
| EPX010-40420-901 | Non-Human Primate Basic Kit | Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay |
| EPX010-20440-901 | Mouse Basic Kit | Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay |
| EPX010-50515-901 | Canine Basic Kit | Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay |
| EPX010-60460-901 | Porcine Basic Kit | Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay |
| EPX010-30420-901 | Rat Basic Kit | Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay |
Platinum kits and panels are designed to overcome matrix effects from serum and plasma samples, thereby improving spike- and dilution-recovery results. Serum and plasma samples are complex matrixes with many components. This complexity can cause the so called matrix effect which can impact the readout of many cytokines (low spike recovery and the dilution linearity). We developed matrix type specific sample diluents to overcome these matrix and multiplex effects, achieving high performance specifications comparable to those of traditional ELISA. These newly developed surrogate matrices for dilution of serum or plasma samples are included in ProcartaPlex Platinum kits, which produce spike- and dilution-recovery results of 70–130%.
Platinum basic kits
| Cat. No. | Name | Description |
|---|---|---|
| EPXP010-10421-901 | Platinum Basic Kit for Plasma Samples | Includes antigen standard, plasma assay diluent, detection antibody diluent, controls, wash buffers, SA-PE and plates - needed to perform the Platinum ProcartaPlex Simplex assay |
| EPXP010-10422-901 | Platinum Basic Kit for Serum Samples | Includes antigen standard, serum assay diluent, detection antibody diluent, controls, wash buffers, SA-PE and plates - needed to perform the Platinum ProcartaPlex Simplex assay |
Platinum panels
| Cat. No. | Name | Antigens |
|---|---|---|
| EPXP420-10200-901 | Platinum ProcartaPlex Panel 1 (42plex) | BDNF, Eotaxin (CCL11), GM-CSF,GRO alpha (KC/CXCL1), HGF, IFN alpha, IFN gamma, IL-10, IL-12p70, IL-13, IL-15, IL-16, IL-17A (CTLA-8), IL-1 alpha, IL-1 beta, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-9, IL-20, IL-21, IP-10, LIF, MCP-2, MIP-1 alpha (CCL3),MIP-1 beta (CCL4), Osteoprotegerin (OPG),PDGF-BB, PECAM-1, P-selectin, RANTES (CCL5), SCF, TNF alpha, TNF-RII, tPA, TSLP, VEGF-A, VEGF-D |
| EPXP130-10100-901 | Platinum ProcartaPlex Panel 2 (13plex) | IFN gamma, GM-CSF, IL-10, IL-12p70, IL-13, IL-17A (CTLA-8), IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8 (CXCL8), TNF alpha |
ProcartaPlex high sensitivity assays are designed to measure small concentration differences in cell culture supernatants, plasma, and serum samples with 10-fold lower LLOQs (Lower Limit of Quantification) and sensitivities for all analytes in the femtogram range. Overcome the sensitivity detection limits of conventional multiplex immunoassays.
ProcartaPlex high sensitivity panels
| HS Panel Name | Cat. No. | Antigen |
|---|---|---|
| 9-Plex Human Panel, High Sensitivity |
EPXS090-12199-901 | IFNγ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12 p70, IL-17A, TNFα |
| 5-Plex Mouse Panel, High Sensitivity |
EPXS050-22199-901 | IFNγ, IL-2, IL-4, IL-6, TNFα |
Human high sensitivity simplex kits
| Simplex Cat. No. | Analyte | ULOQ* (pg/mL) |
LLOQ* (pg/mL) |
Inter Assay %CV |
Intra Assay %CV |
|---|---|---|---|---|---|
| EPXS010-10283-901 | GM-CSF | 7330 | 1.80 | <10 | <10 |
| EPXS010-10228-901 | IFN gamma | 260 | 0.31 | <10 | <10 |
| EPXS010-10224-901 | IL-1 beta | 1330.0 | 0.32 | <10 | <10 |
| EPXS010-10221-901 | IL-2 | 490 | 0.44 | <10 | <10 |
| EPXS010-10225-901 | IL-4 | 560 | 0.86 | <10 | <10 |
| EPXS010-10278-901 | IL-5 | 2720 | 0.66 | <10 | <10 |
| EPXS010-10213-901 | IL-6 | 2850 | 1.03 | <10 | <10 |
| EPXS010-10204-901 | IL-8 | 890 | 0.22 | <10 | <10 |
| EPXS010-10215-901 | IL-10 | 750.0 | 0.18 | <10 | <10 |
| EPXS010-10238-901 | IL-12p70 | 3090.0 | 0.75 | <10 | <10 |
| EPXS010-12017-901 | IL-17A | 1030.0 | 0.25 | <10 | <10 |
| EPXS010-10281-901 | MCP-1 | 650 | 0.16 | <10 | <10 |
| EPXS010-10223-901 | TNF alpha | 1390 | 0.45 | <10 | <10 |
Mouse high sensitivity simplex kits
| Simplex Cat. No. | Analyte | ULOQ* (pg/mL) |
LLOQ* (pg/mL) |
Inter Assay %CV |
Intra Assay %CV |
|---|---|---|---|---|---|
| EPXS010-20606-901 | IFN gamma | 1250.0 | 0.31 | <10 | <10 |
| EPXS010-20601-901 | IL-2 | 1820.0 | 0.44 | <10 | <10 |
| EPXS010-20613-901 | IL-4 | 3530.0 | 0.86 | <10 | <10 |
| EPXS010-20603-901 | IL-6 | 4230.0 | 1.03 | <10 | <10 |
| EPXS010-20607-901 | TNF alpha | 1830.0 | 0.45 | <10 | <10 |
*determined for first lot
ProcartaPlex high sensitivity basic kits
To run simplex kits alone or in combination with other simplex kits, buffer reagents and plate(s) are needed. ProcartaPlex Human and Mouse High Sensitivity Basic Kits contain all necessary support reagents.
| Cat. No. | Description |
|---|---|
| EPXS010-10420-901 | ProcartaPlex Human High Sensitivity Basic Kit |
| EPXS010-20440-901 | ProcartaPlex Mouse High Sensitivity Basic Kit |
Supporting information about ProcartaPlex assays
ProcartaPlex Supplemental Products
Find specialty diluents, buffers, streptavidin-PE conjugate to supplement ProcartaPlex assays, as well as accessory equipment such as magnets and plate washers.
ProcartaPlex Immunoassay Publication List
Browse lists of publications, organized chronologically by year, from peer reviewed journals that cite the use of ProcartaPlex immunoassays.
ProcartaPlex Analyst 1.0 Software
Learn about features of our complimentary multiplex analysis software, free to download with the purchase of ProcartaPlex kits and panels.
Custom Luminex Assays
Use our ProcartaPlex Mix & Match assay option to design your own multiplex assay for detection on Luminex systems. Learn how we can provide custom-blended and optimized panels to deliver results tailored to the design of your choice.
Multiplexing protocols
ProcartaPlex 384-well protocols
ProcartaPlex additional protocols
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For Research Use Only. Not for use in diagnostic procedures.