|
2014-05-22
|
Transfer
EMSA gels using the Pierce G2 Fast Blotter. The Pierce G2 Fast Blotter
provides rapid semi-dry transfer of DNA (TBE) gels to nylon
membrane.
|
Electrophoresis and
Staining, Western Blotting
|
Pierce™
G2 Fast Blotter – Power Stainer Upgrade
|
|
2014-05-19
|
An
improved method for highly efficient isolation of primary mouse embryonic
fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts
(MEF).
|
Lysis and Extraction
|
Pierce™
Mouse Embryonic Fibroblast Isolation Kit
|
|
2014-05-02
|
Pierce
qIP Assay: a novel, HTS-compatible protein-protein interaction assay system.
A cell-based, quantitative co-immunoprecipitation method for protein
interaction analysis involving a sensitive luciferase assay
strategy.
|
Immunoprecipitation,
Luciferase Assays
|
Pierce™
Agarose qIP Protein Interaction Kit, Tluc and HA Tags
|
|
2014-04-02
|
One-hour
procedure to isolate primary cardiomyocytes from neonatal mouse and rat
hearts. Easily isolate and culture highly viable, functional primary
cardiomyocytes.
|
Lysis and Extraction
|
Pierce™
Primary Cardiomyocyte Isolation Kit
|
|
2014-03-24
|
Glycoprotein
expression in a human IVT system. Expression of partially N-linked
glycoproteins using the 1-Step Human Coupled in vitro Translation
System.
|
Protein Expression
|
1-Step
Human Coupled IVT Kit - DNA
|
|
2014-03-24
|
Benchmarking
experiments with Zeba Spin Desalting Columns. Protein recovery performance
comparisons of Thermo Scientific Zeba Spin Desalting Columns to other
commercially available desalting resins and columns.
|
Dialysis and
Desalting
|
Zeba™
Micro Spin Desalting Columns, 7K MWCO, 75 µL
|
|
2014-03-03
|
Calculate
the number of immobilized proteins per bead of agarose affinity supports. A
consideration of relative sizes and dimensions of agarose resin beads,
antibodies, proteins, chemical modification groups, and affinity
ligands.
|
Affinity Purification
|
AminoLink™
Plus Coupling Resin
|
|
2014-02-19
|
Improved,
all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete
Reagent provides gentle, efficient, and cost effective protein extraction
from E. coli and other bacterial cells.
|
Lysis and Extraction
|
B-PER™
Complete Bacterial Protein Extraction Reagent
|
|
2014-01-28
|
An
efficient method for the isolation of highly functional primary neurons.
Easily and reproducibly isolate and culture primary neurons.
|
Lysis and Extraction
|
Pierce™
Primary Neuron Isolation Kit
|
|
2014-01-16
|
Expression
of highly active proteins using a cell-free human IVT system. Active
caspase-3 produced by cell-free protein expression with the 1-Step Human
High-Yield In Vitro Translation Kit is more functional than recombinant
caspase-3 expressed in bacteria.
|
Protein Expression
|
1-Step
Human High-Yield Mini IVT Kit
|
|
2014-01-10
|
System
for high-throughput, high-yield in vitro translation. HTP protein expression
using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis
Plates.
|
Protein Expression
|
1-Step
Human High-Yield Mini IVT Kit
|
|
2013-11-12
|
Luciferase
assays and BioTek Synergy microplate readers. Validation and comparison of
single-step flash and dual-spectral luciferase reporter gene assays using the
Synergy line of microplate readers.
|
Luciferase Assays
|
Pierce™
Renilla Luciferase Flash Assay Kit
|
|
2013-10-22
|
Choosing
a vector and purification method for in vitro protein expression. Strategies
for optimizing expression and purification of functional proteins produced
using the Thermo Scientific 1-Step High Yield IVT protein expression
system.
|
Protein Expression,
Fusion Tag Methods
|
pT7CFE1-NHis
Vector for Mammalian Cell-Free Protein Expression
|
|
2013-09-11
|
Analysis
of androgen-dependent and -independent regulation of transcriptional
activity. Monitor activity using a sensitive and optimized magnetic ChIP
kit.
|
Immunoprecipitation,
Molecular Biology
|
Pierce™
Magnetic ChIP Kit
|
|
2013-08-29
|
Efficient
mammalian membrane protein extraction. Fast and simple enrichment of integral
membrane proteins and membrane-associated proteins.
|
Lysis and Extraction
|
Mem-PER™
Plus Membrane Protein Extraction Kit
|
|
2013-08-19
|
Specific
labeling, enrichment and quantitation of S-nitrosylated peptides using
iodoTMT reagents. Measure protein and peptide cysteine modifications using
multiplex quantitative mass spectrometry.
|
Mass Spectrometry,
Post-translational Modification
|
iodoTMTsixplex™
Label Reagent Set, 1 x 0.2 mg
|
|
2013-08-02
|
Performance
characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic
binding capacity, reusability and chemical compatibility properties of a
glutathione resin for FPLC purification of GST tagged fusion
proteins.
|
Fusion Tag Methods,
Affinity Purification
|
Pierce™
Glutathione Superflow Agarose
|
|
2013-07-11
|
Performance
characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding
capacity, reusability and chemical compatibility properties of a
nickel-charged IMAC resin for FPLC purification of His-tagged
proteins.
|
Fusion Tag Methods,
Affinity Purification
|
HisPur™
Ni-NTA Superflow Agarose
|
|
2013-06-26
|
Improved
Western blot detection using CCD digital imaging. Comparison of cooled-CCD
digital imaging versus X-ray film for sensitivity, dynamic range and signal
linearity with enhanced chemiluminescence (ECL).
|
Western Blotting,
Electrophoresis and Staining
|
myECL™
Imager
|
|
2013-06-24
|
Versatile
luciferases: new tools for reporter assays. Microplate luminometers equipped
with reagent dispensers and optical filters provide optimal performance for
luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase
reporter genes.
|
Luciferase Assays
|
Pierce™
Gaussia Luciferase Flash Assay Kit
|
|
2013-06-05
|
Carrier
protein activation and conjugation data for immunogen preparation.
Experiments with KLH variants and other immunogens to validate solubility,
maleimide activation consistency, peptide conjugation efficiency and
immunogenicity.
|
Antibody Production
|
Imject™
mcKLH (in PBS)
|
|
2013-06-04
|
A
versatile mass spectrometry sample preparation procedure for complex protein
samples. Prepare high-quality peptide digests with high recovery from
cultured cells.
|
Mass Spectrometry,
Sample Preparation
|
Pierce™
Mass Spec Sample Prep Kit for Cultured Cells
|
|
2013-05-22
|
Protocol
for in vitro protein expression using mRNA templates. Use the 1-Step Human
Coupled DNA IVT kit to efficiently translate mRNA templates.
|
Protein Expression
|
1-Step
Human Coupled IVT Kit - DNA
|
|
2013-05-02
|
Performance
characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding
capacity, reusability and chemical compatibility properties of a
cobalt-charged IMAC resin.
|
Fusion Tag Methods,
Affinity Purification
|
HisPur™
Cobalt Superflow Agarose
|
|
2013-04-30
|
Activation
of the antioxidant response pathway by pesticide chemicals. Simultaneous
measurement of the activation of ARE- and NF-κB-driven luciferase reporters
by parkinsonian neurotoxins in neuronal cells.
|
Luciferase Assays
|
Pierce™
Gaussia Luciferase Flash Assay Kit
|
|
2013-04-18
|
Separation
characteristics of dialysis membranes. Molecular weight cut-off (MWCO)
specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis
Devices and Snakeskin Dialysis Tubing.
|
Dialysis and
Desalting
|
Slide-A-Lyzer™
G2 Dialysis Cassettes, 10K MWCO, 3 mL
|
|
2013-03-08
|
Monitoring
neuronal differentiation using multiplexed luciferase reporters.
Characterization of early neuronal differentiation markers in precursor cell
lines using multiplexed luciferase reporters and immunofluorescence
imaging.
|
Luciferase Assays,
IHC and Cell Imaging
|
Pierce™
Cypridina Luciferase Flash Assay Kit
|
|
2013-03-07
|
Chromatography
cartridges for desalting and affinity purification. Data using the cartridge
format for automated liquid chromatography (LC) systems.
|
Affinity
Purification, Dialysis and Desalting
|
Zeba™
Micro Spin Desalting Columns, 7K MWCO, 75 µL
|
|
2013-01-24
|
Simultaneous
dual-emission detection of luciferase reporter assays. Performing
dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate
reader.
|
Luciferase Assays,
Protein Expression
|
Pierce™
Cypridina-Firefly Luciferase Dual Assay Kit
|
|
2012-12-11
|
Quantitative
cancer stem cell phosphoprotein profiling. Using tandem mass labeling and
LC-MS/MS to profile perturbations of an autocrine loop.
|
Mass Spectrometry,
Post-translational Modification
|
Pierce™
Phosphoprotein Enrichment Chromatography Cartridges, 5mL
|
|
2012-11-12
|
Phosphoprotein
enrichment from cell and tissue samples. Isolate phosphorylated proteins with
an easy-to-use spin format
|
Post-translational
Modification, Affinity Purification
|
Pierce™
Phosphoprotein Enrichment Chromatography Cartridges, 5mL
|
|
2012-10-11
|
Cell-free
protein expression system for generating stable isotope-labeled proteins.
Rapidly express protein standards for quantitative mass
spectrometry.
|
Mass Spectrometry
|
1-Step
Heavy Protein IVT Kit
|
|
2012-07-18
|
Eliminate
endotoxins from protein and antibody samples. A highly specific affinity
resin enables endotoxin removal from various protein sources, sizes and
charges.
|
Sample Preparation
|
Pierce™
High Capacity Endotoxin Removal Resin
|
|
2012-07-09
|
Removing
endotoxins using a spin-column format. Fast and effective removal of
endotoxins from protein samples.
|
Sample Preparation
|
Pierce™
High Capacity Endotoxin Removal Resin
|
|
2012-06-13
|
Luciferase
assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with
low transfection efficiency.
|
Luciferase Assays
|
Pierce™
Gaussia Luciferase Flash Assay Kit
|
|
2012-05-18
|
Fractionate
subcellular proteins from tissue samples. Obtain proteins from five cellular
components using a tissue-optimized kit.
|
Lysis and Extraction
|
Subcellular
Protein Fractionation Kit for Tissues
|
|
2012-05-10
|
Method
to isolate functional synaptosomes. Enrich neuronal synaptic proteins while
maintaining phosphoprotein integrity.
|
Lysis and Extraction
|
Syn-PER™
Synaptic Protein Extraction Reagent
|
|
2012-04-18
|
Complete
protein protection from proteases and phosphatases. Protease, phosphatase and
combination inhibitor tablets provide a convenient format to protect proteins
from degradation and dephosphorylation.
|
Lysis and Extraction,
Sample Preparation
|
Pierce™
Protease Inhibitor Tablets, EDTA-free
|
|
2012-03-05
|
Optimized
protein extraction reagent for neuronal tissue and primary cells. Obtain high
yields of functional neuronal protein from all neuronal cell
compartments.
|
Lysis and Extraction
|
N-PER™
Neuronal Protein Extraction Reagent
|
|
2012-02-28
|
Highly
sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and
CRE promoter activity using bioluminescent reporter assays.
|
Luciferase Assays,
Protein Expression
|
Pierce™
Cypridina-Firefly Luciferase Dual Assay Kit
|
|
2012-02-24
|
Using
antibody-binding proteins for antibody purification. Choose a format based on
the specific application and scale.
|
Antibody
Purification, Affinity Purification
|
Pierce™
Protein A Plus Agarose
|
|
2011-10-07
|
Improved
immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide
covalent coupling using a catalyst and an optimized buffer.
|
Immunoprecipitation,
Protein Immobilization
|
GlycoLink™
IP Kit
|
|
2011-06-01
|
Western
blotting using enhanced chemiluminescence. Choose the perfect substrate for
your needs.
|
Western Blotting
|
Pierce™
ECL Western Blotting Substrate
|
|
2011-06-01
|
Serine
hydrolase active-site probes for activity-based enzyme profiling. New
fluorophosphonate probes selectively label and enrich active serine
hydrolases.
|
Protein Labeling,
Protein Assays
|
ActivX™
TAMRA-FP Serine Hydrolase Probe
|
|
2011-06-01
|
Selective
enrichment of sialylated glycopeptides using TiO2 beads. Affinity
purification of glycoproteins for mass spectrometry based on titanium dioxide
beads (TiO2).
|
Mass Spectrometry
|
Pierce™
TiO2 Phosphopeptide Enrichment and Clean-up Kit
|
|
2011-06-01
|
Perform
IP and co-IP experiments with high yield and low background. New Protein A/G
magnetic beads enable manual and automated applications.
|
Immunoprecipitation,
Affinity Purification
|
Pierce™
Protein A/G Magnetic Beads
|
|
2011-06-01
|
Optimize
liquid chromatography with a peptide retention time calibration mixture. New
mixture of 15 heavy peptides streamlines assay design for targeted peptide
quantification.
|
Chromatography, Mass
Spectrometry
|
Pierce™
Peptide Retention Time Calibration Mixture
|
|
2011-06-01
|
Calibration
solutions for Thermo Scientific Mass Spectrometers. Ready-to-use standards
enable quick instrument calibration.
|
Mass Spectrometry
|
Pierce™
LTQ ESI Positive Ion Calibration Solution
|
|
2011-06-01
|
New
dimensions for low-volume sample dialysis. Self-contained devices give you the
solution for high-protein recovery.
|
Dialysis and
Desalting, Sample Preparation
|
Slide-A-Lyzer™
MINI Dialysis Device, 10K MWCO, 0.1 mL
|
|
2011-06-01
|
Introducing
a Western blot substrate that is chemiluminescent and chemifluorescent.
Detect your target by film, CCD cameras or laser-based imagers.
|
Western Blotting
|
Pierce™
ECL Plus Western Blotting Substrate
|
|
2011-06-01
|
Develop
mid- to high-throughput SRM and MRM assays. New application-specific peptide
libraries for quantitative mass spectrometry workflows.
|
Peptides, Mass
Spectrometry
|
PEPotec
SRM Custom Peptide Libraries
|
|
2011-04-05
|
Measure
activation of small GTPases via their specific downstream effectors.
Pull-down kits enrich active GTPases from cell or tissue lysates.
|
Protein Assays,
Immunoprecipitation
|
Active
Rac1 Pull-Down and Detection Kit
|
|
2011-04-05
|
GTPase
enrichment using a new active-site probe. Introducing a desthiobiotin
nucleotide probe for studying GTPases.
|
Protein Labeling,
Affinity Purification
|
Pierce™
GTPase Enrichment Kit with GTP Probe
|
|
2011-04-05
|
Enhanced
immunostaining means more signal and better sensitivity. Achieve better protein
detection using a fraction of your valuable primary antibody.
|
IHC and Cell Imaging
|
Pierce™
Immunostain Enhancer
|
|
2011-04-05
|
Detection
and localization of active GTPases in neuronal cell differentiation. GTPase
pull-down kits enable powerful signal transduction studies.
|
IHC and Cell Imaging,
Affinity Purification
|
Active
Rac1 Pull-Down and Detection Kit
|
