GAPDH Loading Control Antibody (MA5-15738-D800) in WB

Western blot analysis of GAPDH was performed by loading 75ug of the indicated cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product ## 22860) and blocked with SEA BLOCK Blocking Buffer (Product ## 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated GAPDH monoclonal antibody (Product ## MA5-15738-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform, and washed in TBS-0.1% Tween-20. Fluorescent detection was performed using a near IR imaging system.

Western blot analysis of GAPDH was performed by loading 75ug of the indicated cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product ## 22860) and blocked with SEA BLOCK Blocking Buffer (Product ## 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated GAPDH monoclonal antibody (Product ## MA5-15738-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform, and washed in TBS-0.1% Tween-20. Fluorescent detection was performed using a near IR imaging system.

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