iBright Imaging Systems
All-in-one western blot imaging and gel documentation instruments
Capture images and analyze data from your western blots and gels efficiently and easily using the Invitrogen iBright Imaging Systems. These high-performance instruments enhance the imaging experience through advanced automated features and an interface that is easy to use for researchers of all experience levels. Be empowered to take on your research challenges and discover western blot and gel imaging designed to work for you.
Watch video: Invitrogen iBright Imaging Systems—the stunningly easy western blot and gel documentation platform
“The iBright Imager is incredibly user friendly!”
– Rebecca Sinnott DeVaux, SUNY
Open the tabs below to learn more about how our iBright Imaging Systems will help streamline your western blot and gel imaging experience.
Feel right at home—The experience begins with our capacitive LCD touch screen, which responds to inputs like other popular touch screen devices do.
Load and go—Insert samples using the motorized drawer. The interface has a simple, logical layout of functions and features, making iBright Imaging Systems easy to use with minimal training. Workflows are similar between imaging modes—for a smooth imaging experience regardless of sample type.
Hassle-free sample alignment, focus, and zoom—rather than having to open the sample door and repeatedly reposition your sample to achieve proper alignment, iBright Imaging Systems automatically determine the sample position and can rotate samples left or right up to 10° on a mechanically rotating sample stage. Mechanical rotation is preferable to digital rotation, as digital rotation can lead to data alterations.
The iBright Imaging Systems automatically determine if the sample requires zoom in order to maximally utilize the field of view. If imaging a single blot, the camera will move toward the sample (up to 2x zoom). This puts the camera physically closer to the sample, which further maximizes sensitivity, as the light travels a shorter distance to the camera sensor. The iBright Imaging Systems automatically adjust focus for each level of zoom, producing crystal-clear images.
Analysis in a fraction of the time—iBright Imaging Systems feature automatic on-board data analysis, which allows for instantaneous lane and band identification and molecular weight marker overlay, greatly simplifying and streamlining basic post-image data analysis. Quantitation and densitometry analysis can be performed directly on the instrument. Up to 4 blots or gels can be analyzed simultaneously, greatly increasing throughput.
Capture crystal-clear images—Smart Exposure technology rapidly determines optimal exposure time, which helps minimize the potential for over- or underexposed images, and the need to repeat exposures to get the desired signal. Combining Smart Exposure technology with a sensitive 9.1 megapixel (MP) cooled CCD camera, iBright instruments provide powerful imaging potential, which helps enable the detection of subtle differences in protein expression.
Big field of view, small footprint—The iBright Imaging Systems feature a light path design that helps enable a large functional viewing area in a relatively small instrument footprint.
Long-life epi-LED illumination—The iBright FL1000 Imaging System uses a simple combination of two high-quality long-life epi-LEDs for fluorescent imaging applications. One broad spectrum white LED is used as the light source for RGB fluorescence and far-red fluorescence. The other LED is optimized for near-IR fluorescence. Light from these sources passes through our excitation and emission filters to enable many possible reagent options for protein gel, nucleic acid gel, and blot imaging applications (Table 1).
Table 1. Overview of the types of data that can be captured from gels and blots.
|Imaging capability||What kind of signal can be captured?|
|Protein gel||Colorimetric staining of gels (e.g., Coomassie dye, silver) and membranes (e.g., Ponceau S, Thermo Scientific MemCode stain)|
|Nucleic acid gel||Ethidium bromide and Invitrogen SYBR dye staining|
|Chemiluminescent blot||Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)|
|Fluorescent blot*||Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor, Alexa Fluor Plus, Thermo Scientific DyLight dyes)|
|*FL1000 model only.|
Accelerate your research—The iBright FL1000 model features five fluorescence channels, permitting up to 4-color fluorescent western blot multiplexing and expanding your possibilities for studying multiple proteins in a single blot. Obtain meaningful and representative comparisons to enhance your experiments. Smart Exposure technology further improves acquisition of multiplex fluorescent western blot data, because signal-to-noise ratios are optimized for each fluorescent channel separately.
The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer many additional benefits.
No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Furthermore, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
No mercury waste: UV transilluminator bulbs contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
Longer lifetime: LED bulbs have a substantially longer real-time life than UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.
Big performance in a simple package—iBright Analysis Software expands upon the on-board analysis features of the iBright Imaging Systems, with in-depth image adjustment and data analysis capabilities. iBright Analysis Software was designed to complete the overall intuitive imaging experience.
Cloud connectivity helps to increase productivity—iBright Analysis Software is built on the Thermo Fisher Cloud web-based platform, and is part of the Thermo Fisher Connect suite. Data can be exported directly from iBright Imaging Systems and securely stored in Thermo Fisher Cloud. As the iBright Analysis Software is web-based, you can access, review, analyze, and share your data wherever an internet connection is available. Furthermore, with Thermo Fisher Connect you can determine instrument status, firmware edition, and usage history, providing an extra degree of control over the monitoring of your investment.
Keep your data secured—Thermo Fisher Connect is powered by Thermo Fisher Cloud, which uses powerful security standards, including high levels of encryption and network firewalls, so your data remain secure. Thermo Fisher Cloud also provides data backup and recovery so that even in emergencies, your data remain secure.
Fluorescent western blotting - an introduction for new users
A primer to fluorescent western blotting, including protocol, tips & tricks
For Research Use Only. Not for use in diagnostic procedures.