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Robotic Automation for Flow Cytometry
Efficient. Flexible. Transformative.
The Invitrogen Attune NxT Flow Cytometer is configurable for applications benefiting from robotic integration. Customize your Attune NxT Flow Cytometer Robotics Configuration to fully leverage operating capacity, mitigate human operator error and enable rich, reproducible data.
Choose the components you need for robotic plate taxiing, extended fluidics, and temperature-stable plate storage.
Advantages of the Attune NxT Flow Cytometer Robotics Configuration include:
- Reliable—Automated with extended unmanned runtime settings
- Scalable—Build the Attune NxT configuration you need for your applications
- Efficient—Maximized capacity for processing large quantities of data
Reliable operation, innovative mechanical integrity
Multi-component flow cytometry robotics
High-throughput screening for flow cytometry requires multiple instrument components but offer autonomy to the researcher. This video shows how reliable robotic automation enables error-set-up and overnight plate screening runs using unattended mode.
Leverage the Attune NxT Flow Cytometer Robotics Configuration for outstanding performance, walkaway reliability, accurate monitoring, and productive uptime. Optimize for particular specifications, workload, and objectives of multi-instrument, high-volume labs with custom upgrades.
- Rich data—this degree of automation enables measurably more consistency
- Coordinated operations—boost efficiency and trim production cycle intervals
- Sustained reliability—mechanically engineered to achieve long-lasting high-performance
True walk-away automation in biotech and biopharma labs is not achieved solely with the integration of robotics and defined points of user intervention but rather when every instrument in the workcell is reliable. A system’s speed is inconsequential if the system is not also robust in its operation. Choose expertly engineered instruments for your automation workcell.
The components comprising the Attune NxT Flow Cytometer Robotics Configuration include:
- Flow cytometer—speed and mechanical integrity
- Plate sampler—consistent plate sampling
- Expanded fluidics—high-volume fluidics for continuous run time
- Robotic arm—precise microplate taxiing
- Environmental Controls and microplate storage—sequential access stacks, programmed-access hotels, and available inventory management system
Attune NxT Flow Cytometer Robotics Configuration
Attune NxT robotics configuration
Watch a video of the Attune NxT Flow Cytometer configured with robotic integration. Learn about the performance, engineering, safety, and software design of the Orbitor RS2 Microplate Mover.
The Attune NxT Flow Cytometer automation configurability can flex to your needs, sample volume, and sample type.
Attune NxT Flow Cytometer
- Efficient—up to 10x faster than traditional flow cytometric analyzers
- Flexible—up to 14 colors and up to 6 channels with the violet laser
- Transformative—run difficult samples with a system that has active mechanical resistance to clogging
Attune NxT CytKick and CytKick Max Autosamplers
- Low carryover—standard mode <0.5% (two wash cycles); multiple-rinse capability for ultralow carryover
- Thorough sample mixing—each well is mixed via full aspiration (no shaking) to preserve cell viability
- Maximized sampling—small dead volume of 30 μL for 12.5–200 μL/min, 50 μL for 50–1,000 μL/min
Orbitor RS2 Microplate Mover
- Versatile—can evolve with your labs needs
- Efficient—integrated barcode scanner with plate detection in gripper
- Safe—active and passive protective features
Momentum Workflow Scheduling Software
- Capable—instrument compatibility drivers available for over 350 instruments
- Dynamic—create multiple workflows with confidence
- Intuitive—easy to use dashboard coupled with the scheduling interface makes executing workflows simple
- Complete control—precise temperature, humidity and CO2 control with additional optional features
- Proven protection—on-demand ContraCon decontamination routine
- Unmatched speed—average access time of 12 seconds with a dedicated plate shuttle system
Attune NxT External Fluid Supply (EFS)
- Extended fluid options—a 10 L cube increases the period of continual operation by reducing the frequency of replenishing and emptying the fluid
- Active monitoring—waste bottle features a vent, sensor, and fill-line alarm
- Benchtop flexibility—umbilical cords enables stowage either under-the-bench or on an adjacent cart
Table 1. Five configuration options for the Attune NxT Flow Cytometer. Configurations listed with the associated components.
| Attune NxT Flow Cytometer configuration option | Components |
|---|---|
| Base Configuration |
|
| Automation Configuration |
|
| Automation Configuration with Extended Fluidics |
|
| Robotics Configuration |
|
| Robotics Configuration with Sample Management |
|
Technical specifications
Thermo Scientific Orbitor RS2 Microplate Mover
| Axis/Joint |
|
| Grip |
|
| Operation |
|
| Safety |
|
| Physical Characteristics |
|
| Software |
|
| Requisite Environmental Conditions |
|
| Power |
|
| Data Management |
|
| Elevator, Reach and Axes | Clearance (H x W x D)
|
| Microplate Storage |
|
| Mounting | Supporting surface area
|
Thermo Scientific Cytomat 2 C-LiN Automated Incubator Series
| Temperature Range |
|
| Inventory |
|
| Protection |
|
Attune NxT External Fluid Supply (EFS)
| Focusing Fluid |
|
| Waste |
|
| Physical Characteristics |
|
| Operating Range |
|
Engineering of the Orbitor RS2 Microplate Mover
Robotics engineering for flow cytometry workcell
Design thinking behind robotic arm for pharma screening in high-throughput environment. Why the robot is engineered with an integrated barcode reader, bi-directional wrist, and such broad plate type compatibility.
Barcode reading
For sample traceability, plate data storage, and barcode transmission, the Orbitor RS2 mover is designed to synchronize barcodes. The Orbitor RS2 mover has an on-board barcode reader in the grip (Figure 1).
Grip
- Grip force settings based on plate type and weight with sample
- Grip detection
- Integrated plate sensor
- Bi-directional wrist for portrait or landscape clutching
Diverse plate compatibility
- A pre-populated list of plate types
- Additional plate types may be added for a variety of applications
- Lidded plates to and from storage, and un-lid and re-lid plates
Hotels and stacks
- Storage platforms configured with an arrangement of plates and hotels (Figure 2)
- Random-access microplate hotels
- Sequential-access storage stacks
- Landscape and portrait plate orientation
Figure 1. Plate gripper with integrated barcode reader.
Figure 2. Two hotels and two stacks positioned to store and receive well plates. Extended platforms are available for expanded plate capacity.
Straightforward, feature-rich software
Momentum software for flow cytometry automation
Scheduling software for robotic integration for Attune NxT Robotics Configuration automation. How the user defines the application, schedules the screening assay, and prioritizes the plate in a high-throughput lab.
The robotic automation system is managed centrally though the Thermo Scientific Momentum Workflow Scheduling Software. Create, execute, and monitor the workflows of the Orbitor RS2 mover and the Attune NxT flow cytometer with the software that combines power with ease of use.
- Capable—instrument compatibility drivers available for over 350 instruments
- Dynamic—helps you create multiple workflows with more confidence
- Intuitive—easy-to-use dashboard coupled with the scheduling interface is designed to make executing workflows simple
Figure 3. Home screen user interface of the Thermo Scientific Momentum Laboratory Automation Workflow Software.
The Attune NxT software is designed for compatibility with Momentum software (Figure 4). Automation buttons on the ribbon in Attune NxT software control automation settings. When automation is enabled, Momentum software connects the Orbitor RS2 to the Attune NxT Flow Cytometer.
Figure 4. (A) Home screen of the user-interface of the Attune NxT software. (B) Automation icons on the ribbon of the Attune NxT software.
Designed with multiple safety features
Lab robotics safety considerations
This video overviews the active and passive safety technology features expected within the robotic flow cytometry automation workflow. Pharma and biotech labs conducting plate screening require split-second contact response time.
The Attune NxT Robotics Configuration helps enable a safe working environment. The Orbitor RS2 robot was designed with active and passive safety technology features including:
- Rubber grip bumper—an overhead grip with protective layer against jabbing or protruding fingers
- Self-homing mover—the mover will home itself and open up communication with instrument upon system start-up
- Advanced error handling—Unattended Mode increase operator autonomy during extended unattended operation. This mode has "in-advance" error set-up with user-defined escalation indicated by system error beacon (Figure 5). Set email and text notifications to introduce autonomy in your routine and peace of mind during overnight runs.
- Rapid response—the instrument responds with split-second reaction time upon minimal contact
- Manual override mechanism—emergency stop button (Figure 6) manually halts operation; user-initiated stops and last known location logged in the system
Figure 5. System error beacon for advanced error notification with “ignore” “warning” or “error” event distinctions.
Figure 6. Emergency stop device hardwired into the Orbitor RS2 to enable manual operation override. Standard electronic override stopping button ceases all robotic motion is split-second when pressed.
Experienced global Service & Support
Invitrogen Flow Cytometry Service and Support
What to expect for aftermarket care for integrated robotic automation for flow cytometry. This video shows the multiple facets of post-purchase service and support including Field Service Engineering, Tech Assist, post-install Technical Training.
The Invitrogen Flow Cytometry team understands the post-purchase needs of researchers for operation and maintenance, after-market technical service, and ownership of instruments. We work with researchers every day from all over the globe. Are you new to being the point person for the flow cytometer? What is the most difficult sample you need to analyze? Are you prepared to grow this technology within your organization? Invitrogen and Thermo Scientific instruments are supported by an extensive, experienced global team offering solutions to ensure that your Attune NxT Flow Cytometer configuration is optimized for accuracy, productivity, and performance.
Our premier service and support network options include:
Integration and Relocation services
Our Field Service Engineers are available for setting up or relocating a multicomponent workcell or adding additional instruments to the configuration.
Service and Support
Field Service Engineers dispatch to service calls and manage all instrument installs. An extended service option also available. Preventative maintenance is another valuable aspect of your investment.
Technical Support
A tech support line is available with rolling time zone routing. Speak with an engineer or technician on the phone to answer questions and troubleshoot.
Software application support
Take advantage of the nimble adaptability of Momentum Laboratory Automation Workflow Software to configure operational settings that help enable your automated workcell to meet your needs. Our technical specialists are able to collaborate with you to establish and modify software applications and upgrades.
Application specialists
Globally, dozens of PhD scientists build the ranks of our teams for supporting, developing and partnering with you to expand the complexity of your cell-based assays, determine the best instrumentation configuration for your applications and help you just get to the science.
Training workshops
Post-install, labs receive a 4-hour familiarization workshop to learn about operating the Attune NxT flow cytometer in concert with the Orbitor RS2 Microplate Mover, the External Fluid Supply and the SmartStor microplate storage device. Supplementary sessions are available for expanded operation and application optimization.
Reliable operation, innovative mechanical integrity
Multi-component flow cytometry robotics
High-throughput screening for flow cytometry requires multiple instrument components but offer autonomy to the researcher. This video shows how reliable robotic automation enables error-set-up and overnight plate screening runs using unattended mode.
Leverage the Attune NxT Flow Cytometer Robotics Configuration for outstanding performance, walkaway reliability, accurate monitoring, and productive uptime. Optimize for particular specifications, workload, and objectives of multi-instrument, high-volume labs with custom upgrades.
- Rich data—this degree of automation enables measurably more consistency
- Coordinated operations—boost efficiency and trim production cycle intervals
- Sustained reliability—mechanically engineered to achieve long-lasting high-performance
True walk-away automation in biotech and biopharma labs is not achieved solely with the integration of robotics and defined points of user intervention but rather when every instrument in the workcell is reliable. A system’s speed is inconsequential if the system is not also robust in its operation. Choose expertly engineered instruments for your automation workcell.
The components comprising the Attune NxT Flow Cytometer Robotics Configuration include:
- Flow cytometer—speed and mechanical integrity
- Plate sampler—consistent plate sampling
- Expanded fluidics—high-volume fluidics for continuous run time
- Robotic arm—precise microplate taxiing
- Environmental Controls and microplate storage—sequential access stacks, programmed-access hotels, and available inventory management system
Attune NxT Flow Cytometer Robotics Configuration
Attune NxT robotics configuration
Watch a video of the Attune NxT Flow Cytometer configured with robotic integration. Learn about the performance, engineering, safety, and software design of the Orbitor RS2 Microplate Mover.
The Attune NxT Flow Cytometer automation configurability can flex to your needs, sample volume, and sample type.
Attune NxT Flow Cytometer
- Efficient—up to 10x faster than traditional flow cytometric analyzers
- Flexible—up to 14 colors and up to 6 channels with the violet laser
- Transformative—run difficult samples with a system that has active mechanical resistance to clogging
Attune NxT CytKick and CytKick Max Autosamplers
- Low carryover—standard mode <0.5% (two wash cycles); multiple-rinse capability for ultralow carryover
- Thorough sample mixing—each well is mixed via full aspiration (no shaking) to preserve cell viability
- Maximized sampling—small dead volume of 30 μL for 12.5–200 μL/min, 50 μL for 50–1,000 μL/min
Orbitor RS2 Microplate Mover
- Versatile—can evolve with your labs needs
- Efficient—integrated barcode scanner with plate detection in gripper
- Safe—active and passive protective features
Momentum Workflow Scheduling Software
- Capable—instrument compatibility drivers available for over 350 instruments
- Dynamic—create multiple workflows with confidence
- Intuitive—easy to use dashboard coupled with the scheduling interface makes executing workflows simple
- Complete control—precise temperature, humidity and CO2 control with additional optional features
- Proven protection—on-demand ContraCon decontamination routine
- Unmatched speed—average access time of 12 seconds with a dedicated plate shuttle system
Attune NxT External Fluid Supply (EFS)
- Extended fluid options—a 10 L cube increases the period of continual operation by reducing the frequency of replenishing and emptying the fluid
- Active monitoring—waste bottle features a vent, sensor, and fill-line alarm
- Benchtop flexibility—umbilical cords enables stowage either under-the-bench or on an adjacent cart
Table 1. Five configuration options for the Attune NxT Flow Cytometer. Configurations listed with the associated components.
| Attune NxT Flow Cytometer configuration option | Components |
|---|---|
| Base Configuration |
|
| Automation Configuration |
|
| Automation Configuration with Extended Fluidics |
|
| Robotics Configuration |
|
| Robotics Configuration with Sample Management |
|
Technical specifications
Thermo Scientific Orbitor RS2 Microplate Mover
| Axis/Joint |
|
| Grip |
|
| Operation |
|
| Safety |
|
| Physical Characteristics |
|
| Software |
|
| Requisite Environmental Conditions |
|
| Power |
|
| Data Management |
|
| Elevator, Reach and Axes | Clearance (H x W x D)
|
| Microplate Storage |
|
| Mounting | Supporting surface area
|
Thermo Scientific Cytomat 2 C-LiN Automated Incubator Series
| Temperature Range |
|
| Inventory |
|
| Protection |
|
Attune NxT External Fluid Supply (EFS)
| Focusing Fluid |
|
| Waste |
|
| Physical Characteristics |
|
| Operating Range |
|
Engineering of the Orbitor RS2 Microplate Mover
Robotics engineering for flow cytometry workcell
Design thinking behind robotic arm for pharma screening in high-throughput environment. Why the robot is engineered with an integrated barcode reader, bi-directional wrist, and such broad plate type compatibility.
Barcode reading
For sample traceability, plate data storage, and barcode transmission, the Orbitor RS2 mover is designed to synchronize barcodes. The Orbitor RS2 mover has an on-board barcode reader in the grip (Figure 1).
Grip
- Grip force settings based on plate type and weight with sample
- Grip detection
- Integrated plate sensor
- Bi-directional wrist for portrait or landscape clutching
Diverse plate compatibility
- A pre-populated list of plate types
- Additional plate types may be added for a variety of applications
- Lidded plates to and from storage, and un-lid and re-lid plates
Hotels and stacks
- Storage platforms configured with an arrangement of plates and hotels (Figure 2)
- Random-access microplate hotels
- Sequential-access storage stacks
- Landscape and portrait plate orientation
Figure 1. Plate gripper with integrated barcode reader.
Figure 2. Two hotels and two stacks positioned to store and receive well plates. Extended platforms are available for expanded plate capacity.
Straightforward, feature-rich software
Momentum software for flow cytometry automation
Scheduling software for robotic integration for Attune NxT Robotics Configuration automation. How the user defines the application, schedules the screening assay, and prioritizes the plate in a high-throughput lab.
The robotic automation system is managed centrally though the Thermo Scientific Momentum Workflow Scheduling Software. Create, execute, and monitor the workflows of the Orbitor RS2 mover and the Attune NxT flow cytometer with the software that combines power with ease of use.
- Capable—instrument compatibility drivers available for over 350 instruments
- Dynamic—helps you create multiple workflows with more confidence
- Intuitive—easy-to-use dashboard coupled with the scheduling interface is designed to make executing workflows simple
Figure 3. Home screen user interface of the Thermo Scientific Momentum Laboratory Automation Workflow Software.
The Attune NxT software is designed for compatibility with Momentum software (Figure 4). Automation buttons on the ribbon in Attune NxT software control automation settings. When automation is enabled, Momentum software connects the Orbitor RS2 to the Attune NxT Flow Cytometer.
Figure 4. (A) Home screen of the user-interface of the Attune NxT software. (B) Automation icons on the ribbon of the Attune NxT software.
Designed with multiple safety features
Lab robotics safety considerations
This video overviews the active and passive safety technology features expected within the robotic flow cytometry automation workflow. Pharma and biotech labs conducting plate screening require split-second contact response time.
The Attune NxT Robotics Configuration helps enable a safe working environment. The Orbitor RS2 robot was designed with active and passive safety technology features including:
- Rubber grip bumper—an overhead grip with protective layer against jabbing or protruding fingers
- Self-homing mover—the mover will home itself and open up communication with instrument upon system start-up
- Advanced error handling—Unattended Mode increase operator autonomy during extended unattended operation. This mode has "in-advance" error set-up with user-defined escalation indicated by system error beacon (Figure 5). Set email and text notifications to introduce autonomy in your routine and peace of mind during overnight runs.
- Rapid response—the instrument responds with split-second reaction time upon minimal contact
- Manual override mechanism—emergency stop button (Figure 6) manually halts operation; user-initiated stops and last known location logged in the system
Figure 5. System error beacon for advanced error notification with “ignore” “warning” or “error” event distinctions.
Figure 6. Emergency stop device hardwired into the Orbitor RS2 to enable manual operation override. Standard electronic override stopping button ceases all robotic motion is split-second when pressed.
Experienced global Service & Support
Invitrogen Flow Cytometry Service and Support
What to expect for aftermarket care for integrated robotic automation for flow cytometry. This video shows the multiple facets of post-purchase service and support including Field Service Engineering, Tech Assist, post-install Technical Training.
The Invitrogen Flow Cytometry team understands the post-purchase needs of researchers for operation and maintenance, after-market technical service, and ownership of instruments. We work with researchers every day from all over the globe. Are you new to being the point person for the flow cytometer? What is the most difficult sample you need to analyze? Are you prepared to grow this technology within your organization? Invitrogen and Thermo Scientific instruments are supported by an extensive, experienced global team offering solutions to ensure that your Attune NxT Flow Cytometer configuration is optimized for accuracy, productivity, and performance.
Our premier service and support network options include:
Integration and Relocation services
Our Field Service Engineers are available for setting up or relocating a multicomponent workcell or adding additional instruments to the configuration.
Service and Support
Field Service Engineers dispatch to service calls and manage all instrument installs. An extended service option also available. Preventative maintenance is another valuable aspect of your investment.
Technical Support
A tech support line is available with rolling time zone routing. Speak with an engineer or technician on the phone to answer questions and troubleshoot.
Software application support
Take advantage of the nimble adaptability of Momentum Laboratory Automation Workflow Software to configure operational settings that help enable your automated workcell to meet your needs. Our technical specialists are able to collaborate with you to establish and modify software applications and upgrades.
Application specialists
Globally, dozens of PhD scientists build the ranks of our teams for supporting, developing and partnering with you to expand the complexity of your cell-based assays, determine the best instrumentation configuration for your applications and help you just get to the science.
Training workshops
Post-install, labs receive a 4-hour familiarization workshop to learn about operating the Attune NxT flow cytometer in concert with the Orbitor RS2 Microplate Mover, the External Fluid Supply and the SmartStor microplate storage device. Supplementary sessions are available for expanded operation and application optimization.
3D Demo: Explore the Attune NxT Robotics Configuration
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