Ion AmpliSeq NGS Panels for Targeted Sequencing
One tube. Many answers.
Use DNA or RNA for SNP, CNV, fusion, and indel detection
When analyzing suspected variants in genetic diseases, you need a discovery tool that is both rapid and cost-effective for analyzing multiple genes. Ready-to-use, made-to-order, and on-demand Ion AmpliSeq NGS panels can help.
Ion AmpliSeq panels consist of a pool of oligonucleotide primer pairs, each pair designed to amplify a specified genomic region. Each panel can be designed to interrogate all bases across a gene, or it can be focused on specific mutation hotspots.
Gene panels on demand, how and when you want them
Ion AmpliSeq On-Demand panels bring smaller pack sizes and an optimized catalog of pre-tested genes to custom NGS panels, so you can iterate panel design for human disease research with efficiency and convenience.
Not working with human research samples? No problem.
Design a custom panel from 12 predefined genomes, or from your own reference genome after uploading a reference file.
One tube for CNV, SNP, indel, and fusion detection
Simple, versatile technology based on highly multiplexed PCR helps you target tens to hundreds of genes across mutation types including SNPs, indels, fusions, and copy number variants.
What can you do with NGS using Ion AmpliSeq panels? Get results.
After more than 25,000 custom designs and 600 publications, custom Ion AmpliSeq panels have a track record of superior coverage uniformity, reproducibility, and specificity. They help you get results.
Ion AmpliSeq panels
On-Demand panels
Ion AmpliSeq On-Demand panels enable custom panel design in smaller pack sizes from pre-tested genes for practical customization that helps reduce upfront cost and risk.
RNA-seq panels
Choose from over 20,000 genes to survey gene expression fold changes starting with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA.
Made-to-order
For maximum flexibility, simply select a subset of targets and enter your specific genomic content online using the Ion AmpliSeq Designer tool, and we will design and create customized Ion AmpliSeq panels just for you. Choose from a number of reference genomes or upload your own reference sequence.
Transcriptome panels
The Ion AmpliSeq Transcriptome Human Gene Expression Kit is ideal for traditional gene expression analysis of known transcripts from the RefSeq database.
Ready-to-use panels
The convenient predesigned Ion AmpliSeq ready-to-use panels allow researchers to focus on data generation and analysis, not on the labor-intensive primer design and target selection steps.
Exome panels
The Ion AmpliSeq Exome RDY S5 Kit allows for rapid, accurate sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.
Ion AmpliSeq panels by application
Inherited disease research
Deafness, epilepsy, cardiac research, and more
Cancer research
Cancer genomics, cancer RNA expression, and more
Infectious disease research
Tracing the origin of outbreaks, food safety, and more
Human identification
DNA phenotyping, ancestry, and more
Power and speed for targeted NGS analysis
Ion AmpliSeq panels help you get NGS results from more of your challenging FFPE samples. To do it faster, use the Ion S5 System.
Ion AmpliSeq panel results from Boston Children’s Hospital, USA
Applications of targeted NGS panels for clinical research of primary immunodeficiency diseases
Dr. Janet Chou and Wayne Bainter discuss the application of the Ion AmpliSeq Primary Immune Deficiency (PID) Research Panel v2 to accelerate and improve the search for causal variants in clinical research of PIDs.
Vidoes to help you get started
How does Ion AmpliSeq technology work?
Transitioning from Sanger sequencing? It’s easy to get started.
즉시 사용 가능한 맞춤 community 패널을 통한 획기적 변화
| 간편성 | 확장성 | 속도 |
|---|---|---|
| Pool당 10 ng의 DNA | Pool 당 최대 6,144개의 primer | 하루만에 DNA에서 결과 확보 |
| FFPE 호환 가능 | 하나부터 수백개 유전자까지 | 맞춤 패널 설계 2시간 |
| PCR 기반 표적 선택 | Multiplexing을 위한 바코드 96개 | 3.5시간 표적 선택 및 라이브러리 제조 |
미량의 DNA로 가장 광범위한 유전자 분석
Ion AmpliSeq™ 기술은 특정 인간 유전자 또는 게놈 영역의 표적 염기서열분석을 위해 간편하고 빠르게 라이브러리를 구축합니다. Ultrahigh-multiplex PCR에 기초한 Ion AmpliSeq™ technology는 단 10 ng의 DNA 주입으로 여러 유전자를 표적화하여 Ion PGM™ System에서 FFPE 샘플의 염기서열을 분석할 수 있습니다.
다른 표적 선택 방법은 길고 복잡하며 다량의 DNA를 필요로 하기 때문에 이행성연구(translational research) 및 임상 연구에서 흔히 사용하는 포르말린 고정, 파라핀 포매(formalin-fixed, paraffin-embedded, FFPE) 조직에서 DNA 서열을 분석할 수 없습니다. FFPE DNA 단 10ng과 6,144쌍 primer pool 하나만 있으면 AmpliSeq™ Custom workflow를 사용해 하루만에 변이체를 확인할 수 있습니다. 반면, 같은 프로젝트에 다른 workflow를 사용하는 경우에는 100배의 DNA, 8배의 pool, 두 배의 시간이 필요합니다.
광범위한 Ion AmpliSeq™ Panel 중에서 선택할 수 있어 연구에 획기적인 변화를 가져다 줌:
Ion AmpliSeq™ Ready-to-Use Panel
미리 설계된 Ion AmpliSeq™ Ready-to-Use Panel을 사용하면 편리하게 암과 유전병에서 광범위한 유전자 분석이 가능하므로, 연구자들은 힘든 primer 설계와 표적 선택 단계에 초점을 두기보다는 데이터의 생성과 분석에만 주력할 수 있습니다.
Ion AmpliSeq™ Custom Panel
활용도를 최대한 높일 수 있도록 Ion AmpliSeq™ Designer를 사용해 온라인으로 간단히 표적 하위군을 선택하여 특정 genome content를 입력하기만 하면 사용자에게 꼭 맞는 맞춤 Ion AmpliSeq™ Panel이 설계 및 제작됩니다.
Ion AmpliSeq™ Community Panels
최고의 질병 연구자들과 함께 설계한 Ion AmpliSeq™ Community Panel을 통해 유전자 함량을 선택적으로 분석할 수 있을 뿐 아니라, 미리 설계된 community 패널을 맞춤 제작하여 사용자의 특정 연구 요구사항을 충족할 수 있습니다.
Ion AmpliSeq™ Single-day Workflow
Ion AmpliSeq™ Primer pool
Ion OneTouch™ System
Ion PGM™ Sequencer
Torrent Suite 분석 소프트웨어
Ion Reporter™ Software
- Ion AmpliSeq™ Panels, Ion AmpliSeq™ Library Kit, Ion Xpress™ Barcode(multiplexing의 경우), pool당 10 ng의 DNA 샘플을 사용해 라이브러리를 구성합니다.
- 라이브러리를 Ion OneTouch™ System에 전달하여 템플릿을 빠르게 자동 준비합니다.
- 단 1.5-4.5시간 만에 Ion PGM™ Sequencer에서 라이브러리의 서열을 분석합니다. 완벽한 자동화 옵션을 통해 최종 3단계(염기서열 분석 실행, 데이터, 분석, 결과 주석 달기) 작업이 근무시간이 끝나면 자동으로 완료되고 시작합니다.
- Torrent Suite Software를 통해 자동으로 데이터를 분석합니다.
- Ion Reporter™ Software 자동 분석 기능을 사용해 검출된 변이체에 주석을 달고 이를 분석합니다.
Product Literature:
Ion AmpliSeq™ Cancer Hotspot Panel Flyer v2
Ion AmpliSeq™ Cancer Panel Application Note
Ion AmpliSeq™ DNA Custom Panels Flyer
Ion AmpliSeq™ Custom Panels Application Note
Ion AmpliSeq™ Comprehensive Cancer Panel Flyer
Ion AmpliSeq™ Comprehensive Cancer Panel Target Gene List
Ion AmpliSeq™ Inherited Disease Panel Flyer
Ion AmpliSeq™ Inherited Disease Panel Target Gene List
Ion AmpliSeq™ Sample ID Panel Flyer
Additional Resources
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. The content provided herein may relate to products that have not been officially released and is subject to change without notice.