Technical Resources - Media Formulations

Terrific Broth

INTENDED USE: A buffered, enriched medium to obtain high yields of plasmid DNA from transformed E. coli (1). INSTRUCTIONS FOR USE: Suspend 47 g in 1 L of demineralized water. Add 4 ml of glycerol. Autoclave for 15 min at 121°C. RECOMMENDED STORAGE CONDITION: 15°C TO 30°C.

ComponentsMolecular WeightConcentration (mg/L)mM
Inorganic Salts
Dipotassium hydrogen phosphate174.1769.40.0539684
Potassium dihydrogen phosphate136.0862.20.016166247
Other Components
SELECT Peptone 140 (pancreatic digest of casein)11.8Infinity
SELECT Yeast Extract autolyzed low sodium23.6Infinity

Referencia

1. Tartof, K.D. and Hobbs, C.A. (1987) Focus® 9:2, 12.

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