alpha Tubulin Antibody (13-8000) in WB

Western blot analysis of Alpha Tubulin was performed by loading 30 ug of HeLa (lane1), Jurkat (lane2), HepG2 (lane3), A549 (lane4) and Daudi (lane5) lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product ## NP0322BOX), XCell SureLock™ Electrophoresis System (Product ## EI0002), Novex® Sharp Pre-Stained Protein Standard (Product ## LC5800), and iBlot® Dry Blotting System (Product ## IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Alpha Tubulin was detected at 52 kDa using Alpha Tubulin Mouse Monoclonal Antibody (Product ## 13-8000) at 1:250 dilution in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product ## 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

Western blot analysis of Alpha Tubulin was performed by loading 30 ug of HeLa (lane1), Jurkat (lane2), HepG2 (lane3), A549 (lane4) and Daudi (lane5) lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product ## NP0322BOX), XCell SureLock™ Electrophoresis System (Product ## EI0002), Novex® Sharp Pre-Stained Protein Standard (Product ## LC5800), and iBlot® Dry Blotting System (Product ## IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Alpha Tubulin was detected at 52 kDa using Alpha Tubulin Mouse Monoclonal Antibody (Product ## 13-8000) at 1:250 dilution in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product ## 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

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