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Tyrosinase Antibody (356000) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-375 (Lane 1), A-431 (Lane 2), B16-F10 (Lane 3), Caki-1 (Lane 4), Hep G2 (Lane 5) and COLO 205 (Lane 6). The blot was probed with Anti-Tyrosinase Mouse Monoclonal Antibody (Product # 35-6000, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A ~80 kDa band corresponding to Tyrosinase was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product# SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
