TNBSA reaction scheme for detection of primary amines

Trinitrobenzene sulfonate (TNBS) reacts with primary amines molecules to from an orange-colored product, whose absorbance at 335 to 345nm can be measured with a plate reader or spectrophotometer.

Trinitrobenzene sulfonate (TNBS) reacts with primary amines molecules to from an orange-colored product, whose absorbance at 335 to 345nm can be measured with a plate reader or spectrophotometer.

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