SuperBlock Blocking Buffer in PBS is better than milk for sensitive detection of target proteins

HeLa cell lysate (20, 10, 5, 2.5, 1.25, 0.625 and 0.3125µg) was separated by SDS-PAGE, and transferred to nitrocellulose (Panels A-C, Part No. 88014) or PVDF (Panels D-E, Part No. 88585) membrane. Membranes were blocked for one hour using 5% non-fat milk in Tris-buffered saline with 0.05% Tween™ 20 Detergent or SuperBlock Blocking Buffer in phosphate-buffered saline with 0.05% Tween 20 Detergent. Membranes were probed for the indicated targets. Blots were incubated in Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) for 5 minutes and exposed to film (Part No. 34091).

HeLa cell lysate (20, 10, 5, 2.5, 1.25, 0.625 and 0.3125µg) was separated by SDS-PAGE, and transferred to nitrocellulose (Panels A-C, Part No. 88014) or PVDF (Panels D-E, Part No. 88585) membrane. Membranes were blocked for one hour using 5% non-fat milk in Tris-buffered saline with 0.05% Tween™ 20 Detergent or SuperBlock Blocking Buffer in phosphate-buffered saline with 0.05% Tween 20 Detergent. Membranes were probed for the indicated targets. Blots were incubated in Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) for 5 minutes and exposed to film (Part No. 34091).

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