Robust performance against competitors

Amplification curves were obtained for the PGK1 gene over a 6 log dilution series of human universal reference cDNA. Reactions were run in triplicate on a QuantStudio™ 7 instrument under cycling conditions according to manufacturer's recommended protocol. Competitor mixes demonstrated greater inhibition as evidenced by the shape of the curves and the narrowed spacing between the dilution points at the highest cDNA inputs. In addition, several competitor mixes failed to reliably detect the lowest dilution point. Competitor R: Roche (LightCycler® 480 SYBR® Green I Master); competitor B1: BioRad (SsoAdvanced™Universal SYBR® Green Supermix); competitor B2: SsoFast™ EvaGreen® SuperMix; Competitor QB: Quanta Biosciences (PerfeCTA® SYBR® Green SuperMix ); competitor Q: Qiagen (QuantiTect SYBR® Green PCR Kit).

Amplification curves were obtained for the PGK1 gene over a 6 log dilution series of human universal reference cDNA. Reactions were run in triplicate on a QuantStudio™ 7 instrument under cycling conditions according to manufacturer's recommended protocol. Competitor mixes demonstrated greater inhibition as evidenced by the shape of the curves and the narrowed spacing between the dilution points at the highest cDNA inputs. In addition, several competitor mixes failed to reliably detect the lowest dilution point. Competitor R: Roche (LightCycler® 480 SYBR® Green I Master); competitor B1: BioRad (SsoAdvanced™Universal SYBR® Green Supermix); competitor B2: SsoFast™ EvaGreen® SuperMix; Competitor QB: Quanta Biosciences (PerfeCTA® SYBR® Green SuperMix ); competitor Q: Qiagen (QuantiTect SYBR® Green PCR Kit).

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