Peptide mapping is the gold standard for in-depth characterization of biotherapeutics; however, sample preparation, LC runtime and data analysis can be labour intensive and time consuming. Top or middle-down mass spectrometry experiments show promise as an alternate technique for primary order structure confirmation. However, historically top or middle-down characterisation has been limited by bottlenecks in data processing, and lower sequence coverage.
In this webinar, we show how rapid high sequence coverage of mAbs and ADCs can be achieved with advanced top-down fragmentation capabilities such as Higher-energy Collision Dissociation (HCD), Electron Transfer Dissociation (ETD) and Ultra-Violet Photo-Dissociation (UVPD). In addition, we show that the technique can pinpoint sites of oxidation resulting from oxidative stress. Finally, learn about advances in automated software processing that easily map the protein structure and provide confirmation of sequence coverage and process induced modifications.
Join experts from University of Strasbourg and Thermo Fisher Scientific to learn about the latest technology, software and research applications for this highly promising technique.
Learning objectives of webinar
- Learn how high top or middle-down sequence coverage can be obtained by combining multiple modes of fragmentation.
- Learn about new innovations in mass spectrometry and data processing software for top or middle-down characterization.
- Learn how middle-down experiments could be a valuable alternative assay to peptide mapping for fast amino acid sequence confirmation.