App Note: Automated Workflow for mRNA In Vitro Transcription (IVT)

The development of mRNA vaccines is an exciting area of research. However, in vitro transcription (IVT) and purification of mRNA can be a challenging workflow.

This application note describes a fully automated, end-to-end workflow for mRNA IVT, spanning plasmid purification, template preparation, RNA synthesis, and downstream purification. The workflow incorporates integrated quality control strategies—including Sanger sequencing—to confirm nucleic acid identity, assess purity, and enable accurate quantification. The InnoviGene Suite is central to this process, providing robust analysis and interpretation of sequencing data to support reliable, efficient development of mRNA therapeutics.

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