Antibody Purification Using Protein A
Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS™ MabCapture™ A Select Resin features a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.
We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.
- Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
- High performance—resins are designed to maximize protein yield and reduce background
- Economical—pricing is similar to or better than our leading competitors
Choose the right Protein A product for antibody purification
Product links in the last row of each column
Protein A Magnetic Beads
Protein A Agarose
Protein A Plus Agarose
Recombinant Protein A Agarose
MabCapture A Select
|Bead or resin size||2.8 μm||45–165 μm||45–165 μm||45–165 μm||50 μm|
|Binding capacity||25–30 µg human IgG/mg beads||12–19 mg human IgG/mL resin||34 mg human IgG/mL resin||15 to 17 mg human IgG/mL resin||≥37 mg/mL human IgG/mL resin|
|Maximum linear flow rate||N/A||700 cm/hr||700 cm/hr||700 cm/hr||3000 cm/hr|
|Support||Monosized, super paramagnetic polystyrene beads||Crosslinked 6% beaded agarose||Crosslinked 6% beaded agarose||Crosslinked 6% beaded agarose||Polystyrene-divinylbenzene|
|Recommended application scale||screening||batch, pilot||screening, batch||batch||batch, pilot, process|
|Formats available||loose beads||loose beads, chromatography cartridges||loose beads, pre-packed spin columns and kits, 96-well filter plates||loose beads||loose beads|
(1 mL cartridge)
(5 mL cartridge)
For Research Use Only. Not for use in diagnostic procedures.